Cytotoxicity responses to selected ESAT 6 and CFP 10 peptides in tuberculosis英文文献.pdfVIP

Cellular Immunology 265 (2010) 146–155 Contents lists available at ScienceDirect Cellular Immunology journal homepage: /locate/ycimm Cytotoxicity responses to selected ESAT-6 and CFP-10 peptides in tuberculosis M. Madhan Kumar, Alamelu Raja ⇑ Department of Immunology, Tuberculosis Research Center (ICMR), Mayor V.R. Ramanathan Road, Chetput, Chennai 600 031, India a r t i c l e i n f o a b s t r a c t Article history: Cytotoxicity responses were studied for the ESAT-6 peptides Esp1, Esp6, Esp7, Esp8, and CFP-10 peptides, Received 6 February 2010 Cfp6, Cfp7, Cfp8, Cfp9 (synthetic 20-mer peptides) and the recombinant ESAT-6, CFP-10 proteins. Cyto- Accepted 13 August 2010 lytic molecules perforin, granzymes A and B, granulysin responses in healthy household contacts Available online 20 August 2010 (HHC) and pulmonary tuberculosis patients (PTB), were studied by intracellular flow cytometry. Func- tional cytotoxicity was studied in both the groups for the peptides Esp6 and Cfp8 by an enzyme (lactate Keywords: dehydrogenase) based assay. The results revealed that cytolytic molecule positive CD4+ and CD8+ T cells Cytotoxicity were increased in HHC in response to Esp1, Esp6, Cfp8 and Cfp9 immunogenic peptides compared to PTB. Perforin Functional cytotoxicity results showed higher cytotoxicity (not statistically significa