Pilot Scale Production of Fatty Acid Ethyl Esters by an Engineered Escherichia coli Strain Harboring the pMicrodiesel Plasmid英文文献.pdfVIP

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Pilot Scale Production of Fatty Acid Ethyl Esters by an Engineered Escherichia coli Strain Harboring the pMicrodiesel Plasmid英文文献.pdf

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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2010, p. 4560–4565 Vol. 76, No. 13 0099-2240/10/$12.00 doi:10.1128/AEM.00515-10 Copyright © 2010, American Society for Microbiology. All Rights Reserved. Pilot-Scale Production of Fatty Acid Ethyl Esters by an Engineered Escherichia coli Strain Harboring the p(Microdiesel) Plasmid Yasser Elbahloul† and Alexander Steinbu¨chel* Institut fu¨r Molekulare Mikrobiologie und Biotechnologie, Westfa¨lische Wilhelms-Universita¨t Mu¨nster, D-48149 Mu¨nster, Germany Received 25 February 2010/Accepted 27 April 2010 Fatty acid ethyl esters (FAEEs) were produced in this study by the use of an engineered Escherichia coli p(Microdiesel) strain. Four fed-batch pilot scale cultivations were carried out by ﬁrst using glycerol as sole carbon source for biomass production before glucose and oleic acid were added as carbon sources. Cultivations yielded a cell density of up to 61 3.1 g of cell dry mass (CDM) per liter and a maximal FAEE content of 25.4% 1.1% (wt/wt) of CDM. Fuel consisting of methyl esters of fatty acids (FAMEs) and (ADH) (EC ) catalyzing the NADH-dependent reduc- ethyl esters of fatty acids (FAEEs), also referred to as “biodie- tion of acetaldehyde to ethanol (28, 47). Ethanol is produced sel,” is made from renewable farm products such as vegetable under aerobic conditions in substantial amounts that are suf- or animal oils, fats, or recycled cooking greases that are trans- ﬁcient for the transesteriﬁcation process to yield enough esteriﬁed using methanol derived from fossil fuel or bioethanol FAEEs. An acyltransferase (encoded by atfA) is the key en- from