地瓜叶总黄酮的超声辅助提取及生物活性研究-河南农业科学.pdfVIP

2012-12-05 地瓜叶总黄酮的超声辅助提取及生物活 性研究 * 许瑞波 ，周洪英，陈林，曾艳霞，杨树平，胡喜兰 (淮海工学院化工学院，江苏 连云港， 222005) 摘 要：以地瓜叶为原料，采用超声波辅助的方法，通过单因素和正交实验优化了红薯叶总黄酮(Flavonoids form Ipomoea batatas Lam leaf, FIBL)的提取工艺条件，并且研究了FIBL 的体外抗氧化活性和抑菌作用。结 果表明，从地瓜叶中提取总黄酮的最佳工艺条件是：乙醇浓度为 75%，料液比为 1:30 （g:mL ），超声提取 40 min ，然后 70 °C 水浴加热回流提取 50 min，提取2 次，提取率为 6.03% 。用紫外光谱和红外光谱对提取 产物进行表征，证明产物 FIBL 具备黄酮类化合物的基本光谱特征。此外，抗氧化实验结果表明，FIBL 对 超氧阴离子自由基和 DPPH 自由基具有一定的清除能力，但不如 VC 的效果好。抑菌实验结果表明 FIBL 对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌具有良好的抑制作用。 关键词：地瓜叶；黄酮类化合物；提取；抗氧化活性；抑菌作用 Study on Ultrasonic Technique assisted Extraction Technology and Bioactivities of Flavonoids from Ipomoea batatas Lam Leaf XU Rui-bo ，Zhou Hong-ying, Chen Lin, Zeng Yan-xia, Yang Shu-ping, HU Xi-lan (Huaihai Institute of Technology, School of Chemical Engineering, Lianyungang 222005, China) Abstract ：The ultrasonic technique assisted extraction technology of flavonoids from Ipomoea batatas Lam leaf (FIBL) was studied by single factor experiment and orthogonal experimental design. And the antioxidant activity and bacteriostatic action of FIBL in vitro were investigated. The results showed that the optimum extraction technology of FIBL was as following: ethanol concentration of 75%, ratio of solid to liquid of 1:30 (g:mL), extraction time with ultrasonic technique of 40 min, then extract time of 50 min under reflux in water bath of 70 °C, extraction times of 2. And the yield of FIBL is 6.03%. The extract was characterized by UV-vis and IR spectra which indicated that the extracts FIBL possessed common spectral character of flavonoids. Furthermore, the antioxidant experiment results showed that FIBL has certain scavenging capacity to superoxide anion radical and DPPH radica