阿托伐他汀对血脂异常兔血清和脂肪组织脂联素的影响word论文.docxVIP

结论阿托伐他汀能上调血脂异常兔脂肪组织PPARγmRNA的表达从而增加APNmRNA表达，而升高脂肪组织和血清脂联素的分泌水平，这可能是阿托伐他汀抗动脉粥样硬化作用的多效性机制之一。关键词：血脂异常兔；阿托伐他汀；脂联素；过氧化物酶增殖体激活受体γ2TheinfluencesofatorvastatinonlevelsofadiponectininserumandadiposetissueofdyslipidemiarabbitsAbstractObjective:ObservationofthechangeonserumAPNlevelsandPPARγandAPN secretioninadiposetissuebyatorvastatininterventionindyslipidemiarabbit,and tryingto elaborateanti-atherosclerosis mechanismof atorvastatin.Methods:The15NewZealandwhiterabbitswererandomlydividedintothree groups:(1)controlgroup(n=5):ordinaryfeeddietfor8weeks,(2)thehigh cholesterolgroup(n=5):highcholesteroldietfor8weeks,(3)theatorvastatingroup (n=5):highcholesteroldietfor4weeks,inthecontinuedfeedingwithhigh cholesteroldietbasedonthegivenatorvastatindrugfeeding2.5mg?kg-1?D-14weeks. Aftertheendoftheexperiment,wetaketherabbitaorticspecimensandbiopsiesto doHEstaining.Observationonatheroscleroticplaquechanges,obtainedfromthe inguinalsubcutaneousadiposetissueweight,andincubationofadiposetissue. Applicationofreversetranscriptasepolymerasechainreaction(RT-PCR)determinatefatcellsPPARγmRNAandAPNmRNAexpression,enzymelinkedimmunosorbent assay(ELISA)wasmeasuredinserumandinguinalfatcellextractsandconcentration ofAPNusingreversetranscription PCR (RT-PCR ).Results:1Rabbitaorticatheroscleroticplaque areainatorvastatingroup (4.9±0.28mm2)wassignificantlylowerthantheplaqueareainhighcholesterolgroup（6.2±0.43mm2，P0.05).2 Serum low density lipoprotein cholesterol (LDL-C),serumtotalcholesterol(TC)concentrationsinhighcholesterolgroupwere significantlyhigherthanthosein control group(P0.05). Atorvastatin drug interventionfor4weekscansignificantlyreducetherabbitserumLDL-C,TC concentration(comparisonbeforeandaftertreatment,P0.05).3SerumAPN concentrationandAPNconcentrationinfatcellextractinhighcholesterolgroupis lowerthanthosein control group(P0.05).Atorvastatindruginterventionfor43weeksmarkedlyelevatedserumAPNconcentrationandfatcellextractAPN concentration(comparedwithhighcholesterolgroup,P0.05).4APNconcentration