灯盏乙素纯化工艺分析word论文.docxVIP

The Study of the Purification Process of Scutellarin AbstractObjective: In this paper, the research focused on separation process of scutellarin. The optimum condition of scutellarin was found by studying the recrystallization and column chromatography purification effects.Methods: In order to improve the separation and purification technology of scutellarin, we studied the method of recrystallization and column chromatography. The detecting method of scutellarin establish. Different conditions were tested with HPLC and TLC, and determined. By the way of the single factor experiment and orthogonal experimental design, analyzed the factors that affect the the scutellarin purity and yield of recrystallized: the amount of ethanol, the volume fraction of ethanol, standing time and pH. We also compared the impact of different fillers on the column chromatography purification yield and purity of scutellarin in the column chromatography and determine the best method of purification.Results: According to the experiments, the result shows that the optimal scutellarin separation conditions were: ratio of material to liquid, 1:120 (g:mL), Standing time, 40min, ethanol content, 80%, pH=1, The yield of scutellarin contains flavonoids with a purity of over 98% by analysis of HPLC. Besides, Column chromatography purification was also used, its optimum conditions is respectively: silica gel, 200-300 mesh, dichloromethane to methanol, 5:1(v:v).Conclusion: By the repeatability experiments, the methods of the content detecting and purification methods have satisfactory precision, stability and repeatability. It can provide to control the quality of scutellarin and to ensure efficacy2and safely use in clinic. The research for providing scientific basis of further exploitation.Postgraduate: Jun-Hui Xiao Directed by: Professor Xing ZhengProfessor Yu-Sheng ZhouKeywords: scutellarin; purification; recrystallization; column chromatography3前言灯盏细辛又名灯盏花 ， 是菊科植物短葶飞蓬 Erigeron brevisc