甘草酸对纳米氧化锌导致人皮肤细胞光损伤保护作用word论文.docxVIP

AbstractGlycyrrhizic acid is one of the main whitening active ingredients of licorice (Glycyrrhiza). Studies have suggested that glycyrrhizic acid has shown anti- inflammatory, anti-oxidant, melanin inhibition, free radicals scavenging, antiviral and anticancer activities. Glycyrrhizic acid solution, which is quite photo stable and thermal stable, is used for the treatment of inflammation of the skin caused by intense sunlight. If it was added to skin care production or sunscreen cosmetic, its effectiveness could be ensured in some extent.Vitamin C is a accepted water-soluble antioxidant, with good anti-oxidation, anti- freeradicals, to inhibit tyrosinase activity, to deoxidate the skin pigment melanin and to promote collagen growth properties. It is also a common ingredient in skin whitening products. However, its water-solution is very unstable, which is easily oxidized in hot environments or light conditions. Thus limited the application of vitamin C in skin care production or sunscreen cosmetics.This study, the human keratinocyte cells (HaCaT cells) was used as a skin cell modelto determine whether glycyrrhizic acid could protect human skin cells against photo-damage caused by nano-zinc oxide nanoparticles under UVB/UVA irradiation, and the presence of nitrite. First, the stability of glycyrrhizic acid PBS-solution was determined by HPLC. Then, the cell viabilities under different conditions were assayed by MTT method. Finally, protein tyrosine nitration in HaCaT cells was detected by immunofluorescence experiments and Western blotting.The results showed that glycyrrhizic acid PBS-solution was nice photo-stability andthermal stability; glycyrrhizic acid can do dependently protect human skin cells against photo-damage induced by nitrite、nano ZnO-50 and UV irradiation system. However, when nano ZnO-50 was replaced by nano ZnO-90, the protection was lost. These results suggested that glycyrrhizic acid had protective effects human skin cells photo-damag