GeneReplicationandExpression幻灯片.ppt

Part 2 Gene Replication and Expression;What’s Gene?How dose a gene express?;Section 1. DNA Replication;一、Replication Model(s);Cut ;;一、Replication Model(s);Origin 复制起点;二、Control of replication-- in Prokaryote;;质粒ColEⅠ：反义RNA对复制的调控;三、Control of replication-- in Eukaryote;Replication regulation in Eukaryote;小结;关于DNA的自我复制 vs. 复制？;Section 2. Transcription 转录;Recognition of template Initiation;Ⅰ. Transcription in Prokaryote Ⅱ. Transcription in Eucaryote ;Ⅰ. Transcription in Prokaryote （原核生物）;RNA polymerase （RNA聚合酶）;;+1;Promoter （启动子） ;Initiation of transcription;+1;σ;σ: presents specificity on recognition not only of promoter region but also of different gene.;Template DNA;Two patterns: ρ- independent termination ρ –dependent termination;不依赖ρ因子的终止子;反向重复的GC丰富区;transcript;Two patterns: ρ- independent termination ρ –dependent termination;依赖ρ因子的终止子;Two patterns: ρ- independent termination ρ –dependent termination ;小结：;Ⅱ. Transcription in Eucaryote;RNA polymerase：3种;rRNA gene cluster (except 5s rRNA gene);rRNA gene cluster (Pol Ⅰgene );Class Ⅰ promoter(for RNA PolⅠ);Transcription Initiation of Pol Ⅰgene;all genes encoding protein, some encoding U-RNA;Typical mRNA gene (Pol Ⅱ gene);Class Ⅱ Promoter (for RNA Pol Ⅱ) ;TATAAAA;Note : 1. A certain promoter contains any of above elements; however , most promoters lack one of them at least. 2. In some cases , TATA box and initiator form the basal promoter with function in low level. The up- and down-stream elements may enhance or inhibit the function of basal promoter. ;Promoter of SV40;;Enhancer / Silencer;Transcription initiation of Pol Ⅱ gene;Basal transcriptional initiation complex;Activated transcriptional initiation complex;5s rRNA gene, tRNA gene;Pol Ⅲ gene;tRNA gene;TF ⅢC;;3种基因类型的转录启动的比较;真核生物中与转录终止有关的序列;小结;Section 3. Post-transcriptional processing转录后加工;rRNA processing;tRNA processing;;mRNA processing;1).