[生物学]武汉大学09年《基因的分子生物学》讲义2009 summary.pptVIP

* Nucleic acids techniques: Electrophoresis; Restriction digestion; Hybridization (southern, northern, clony); PCR amplification (regular, RT-PCR, mutational PCR); Sequencing (Sanger), genome sequencing (Shotgun and genome assembly), genomic analysis (protein gene identification in bacteria and animals, BLAST search to predict the function of a new protein) DNA cloning and gene expression. (How to analyze a clone, and how to make a fusion protein) * Protein techniques: Protein purification: column chromatography and affinity chromatography; Protein separation by PAGE and identification by western blot Protein sequencing (Edman degradation and tandem mass spectrometry); Proteomics (combine 2-D gel separation and tandem MS ). Study the interactions: Protein-nucleic acids in vitro: Gel retardation Dnase I footprinting Protein-DNA in cells: Chromatin IP Protein-protein: yeast two-hybrid, Co-IP Determining the Structure of protein and nucleic acids: X-ray crystallography, NMR Summary of Molecular Biology course2009 Feb-June 课程内容Contents Molecular Biology of the Gene, 5/6/E Watson et al. (2004/2007) Part I: Chemistry and Genetics Part II: Maintenance of the Genome* Part III: Expression of the Genome*** Part IV: Regulation*** Part V: Methods** The revised central dogma RNA processing The structure of DNA and RNA 基因组的保持 基因组的表达 ***** 学时分配和教学日历 内容 学时 课程简介－从相互认识到中心法则 6 DNA和RNA的结构 3 基因组的保持：DNA复制 6 基因组的表达：转录 （小考1） 6 基因组的表达： RNA剪接与转录后加工 6 基因组的表达：翻译与遗传密码（文献报告开始准备） 6 基因调控：原核调控 3+1.5 基因调控：真核调控1 3+1.5 基因调控：真核调控2－RNA干扰与miRNA调控 3 分子生物学技术 6 平时小考1次/ 文献报告（4天） 3+4天 教学理念For future  LOVE Shaping the future of my students/the world in Wuhan University We have worked together closely and hard to prepare you to meet today’s challenges and become leaders of the future. 世界是平的，英语是当今的国际语言。你们的英语能力提高了。 A language means more opportunities 创新是灵魂，你们发现问题和解决问题的能力提高了。 Identify the problem and resolve it in a proper and efficient way. 团队(爱)让人坚固，你们的团队意识增强了。Team work is an enjoya