桑树氨基环丙烷-羧酸氧化酶基因MnACO启动子功能-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2017, 43(6): 839848 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2017.00839 桑树1-氨基环丙烷-1-羧酸氧化酶基因(MnACO)启动子功能分析 余 建 刘长英 赵爱春 王传宏 蔡雨翔 余茂德* / , 400715 : 1- -1-(ACO), 1- -1-(ACC) MnACO , pMnACO::GUS GUS GUS PCR MnACO1 MnACO2 , 1518 bp 1429 bp, TATA-boxCAAT-box GUS MnACO GUS ; MnACO1 GUS , MnACO2 ; MnACO2 GUS MnACO1 MnACO2 GUS , MnACO1 GUS , MnACO2 GUS qRT-PCR 2 MaACO1 MaACO2 , MaACO MnACO GUS , MnACO , MnACO1 , MnACO2 MnACO1 , ; MaACO2 , : ; ; ACO; ; GUS; Functional Analysis of 1-Aminocyclopropane-1-carboxylate Oxidase Gene’s Promoter in Mulberry * YU Jian, LIU Chang-Ying, ZHAO Ai-Chun, WANG Chuan-Hong, CAI Yu-Xiang, and YU Mao-De College of Biotechnology, Southwest University / Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Chongqing 400715, China Abstract: 1-Aminocyclopropane-1-carboxylate oxidase (ACO) as a key enzyme catalyzes the reaction from 1-Aminocy- clopropane- 1-carboxylate (ACC) to ethylene. In order to explore the function of MnACO in mulberry growth and development and resisting external stresses, we constructed pMnACO::GUS fusion and transformed it into Arabidopsis thaliana , and used GUS histochemical staining to identify GUS activities of transgenic Arabidopsis