[工程科技]ncode_express_sybrgreener_man.pdfVIP

™ ™ NCode VILO miRNA cDNA Synthesis Kit and EXPRESS SYBR® GreenER™ miRNA qRT- PCR Kits Catalog nos. A11193-050, A11193-051, and A11193-052 Version A 12 February 2009 A11176 User Manual ii Table of Contents Kit Contents and Storage v Additional Products vi Overview 1 Instrument Compatibility 7 Methods8 Poly(A) Tailing and cDNA Synthesis 8 General qPCR Guidelines and Parameters 12 qPCR SuperMix Universal—Guidelines and Protocols 15 qPCR SuperMix with Premixed ROX—Guidelines and Protocols 20 Troubleshooting 22 Appendix 24 Technical Support 24 Purchaser Notification 26 References 28 iii iv Kit Contents and Storage Kit This manual is for use with the kits listed below. Each kit is Components shipped on dry ice. Store all components at –20°C for long- and Storage term storage. EXPRESS qPCR SuperMixes may be stored at 4–8°C for up to one month. NCode™ VILO™ miRNA cDNA Synthesis Kit (A11193-050) includes components for 50 cDNA synthesis reactions from miRNA, plus the Universal qPCR Primer. Other qPCR reagents must be ordered separately. ® 10X SuperScript Enzyme Mix 100 μl 5X Reaction Mix 200 μl 10 μM Universal qPCR Primer 250 μl DEPC-Treated Water 2 ml NCode™ ® ™ EXPRESS SYBR GreenER miRNA qRT-PCR Kit Universal (A11193-051) includes components for 50 cDNA synthesis reactions and 200 qPC