光质对离体诱导棕色棉纤维基因表达影响的cDNA-SRAP分析精品.pdfVIP

花 学 报 Cotton Science 20 14 26 渊1冤院34~40 质对离体诱导棕色棉纤维基因表达影响的cDNA-SRAP 分析 1 1 1 1* 1 1,2 1 陈沙沙 樊洪泓 关蕾 林毅 蔡永萍 钱森和 孙旭 1.安徽农业大学生命科学学院袁 徽合肥230036 曰2.安徽工程大学袁 徽芜湖241000冤 摘要院以离体培养棕色棉纤维 暗培养10 d冤为材料袁分别用红尧黄尧蓝尧白光进行处理袁在24 h 光照条件下培养10 d 后袁利用cDNA-SRAP 技术对其基因表达进行分析遥 对其中16 个差异片段测序后进行同源性比对和功能分析 表明院2 个序列功能与植物抗逆性有关 CNGC5 类似蛋白基因尧 +冤-啄- 杜松烯合成酶冤曰3 个序列与生物代谢相关 茁-1,3- 葡聚糖酶13 基因尧质膜胆碱转运蛋白基因尧tau 类谷胱甘肽转移酶基因冤遥其中袁谷胱甘肽转移酶基因与原 花青素合成前体物质的转运有关袁光质可能通过影响谷胱甘肽转移酶基因进而对原花青素的合成产生影响遥 关键词院离体培养曰cDNA-SRAP 曰谷胱甘肽转移酶曰植物抗逆性 中图分类号院S652.035.3 文献标志码院 文章编号院1002-7807 20 14冤01-0034-07 Chen Shasha, Fan Honghong, Guan Lei, Lin Yi, Cai Yongping, Qian Senhe, Sun Xu (1. 230036 2. 24 1000, ) Fibers of brown cotton were treated with different light qualities (red, yellow, blue, and white) for 10 days in 24-h light conditions, after which there gene expressions were analyzed using the cDNA-SRAP technique. The biological functions of 16 differentially expressed genes were predicted and characterized ．The results showed that two fragments had high sequence simi- larity with resistance genes (CNGC5-like protein gene, (+)-delta-cadinene synthase (cdn 1-C6) pseudogene), 3 sequence related to biological metabolism (sugar transporter/spinster transmembrane protein (PtrOATP2)), beta-1,3-glucanase 13(B 13G_ 13) mR- NA, and tau class glutathione transferase GSTU43 ). Among them, the glutathione transferase was associated with the synthesis of proanthocyanidins. Light quality may affect glutathione transferase, thereby affecting the synthesis of proanthocyanidins. culture; cDNA-SRAP; glutathione transferase; stress in plants 棕色 是一种纤维本身呈棕色的彩色 目 体 且在未发育成 纤维的种皮细胞中合成遥 [1] 前棕色棉色素成分尚不清楚遥 肖月华等 利用cD-