兴凯湖梅花鹿TGF-β1基因多态性与茸性状_相关性研究学士论文精品.docVIP

学士学位毕业论文 兴凯湖梅花鹿TGF-β1基因多态性与茸性状 相关性研究 学生姓名：宋双凤 指导教师：李 馨 所在学院：动物科技学院 专 业：动物科学 中国·大庆 2011 年 1 月兴凯湖梅花鹿TGF-β1基因多态性 与茸性状相关性研究 摘 要：以梅花鹿的TGF-β1作为候选基因,分析该基因对梅花鹿产茸量性状的影响。以兴凯湖梅花鹿为实验群体，PCR-SSCP)和DNA测序的方法检测了TGF-β1基因单核苷酸多态性(SNPs), 针对该群体的特点建立合适的统计分析模型,并进行了TGF-β1基因多态性与产茸量的相关分析。结果表明, 3种基因型间的第二锯产茸量存在一定的差异(P0.05),AA基因型个体在第二锯的产茸量与AB基因型个体之间有一定的差异(P0.05)。 关键词：TGF-β1基因；兴凯湖梅花鹿 ；单核苷酸多态；产茸量性状 Xingkai lake sika deer TGF - β1 gene polymorphism and chopped properties dependence research Abstract：In the TGF–β1 sika deer as candidate genes, analysis of the gene for sika deer production quantity traits. The influence of chopped With xingkai lake sika deer for experimental group, using single-strand conformation polymorphism (PCR - SSCP) and DNA sequencing method to test the TGF–β1 gene single-nucleotide polymorphisms (SNPs), according to the group characteristics to establish appropriate statistical analysis model, and the TGF–β1 gene polymorphism with production chopped amount of correlation analysis. The results showed that three between different genotypes in second saws chopped quantity there exist certain differences (P 0.05), AA genotype individual in the second saws in volume and saut AB genotype between individuals have certain difference (P 0.05). Keywords: TGF–β1 gene, Xingkai lake sika deer, SNP, antler production trait 目 录 前言......................................................1 1 材料与方法..............................................1 1.1 材料..................................................1 1.2 方法..................................................2 2 结果....................................................3 2.1 血液基因组DNA检测...................................3 2.2 PCR产物检测..........................................4 2.3 聚丙烯酰胺凝胶分离PCR产物............................5 2.4 TGF-β1基因各基因型的序列比对..........................5 2.5 TGF-β1基因多态性的等位基因的基因型频率................5 2.6 TGF-β1基因