运动训练对D-半乳糖造阿尔茨海默病模型大鼠海马-中国康复医学杂志.PDFVIP

2012年，第27卷，第2期 ·基础研究· 运动训练对D-半乳糖造阿尔茨海默病模型大鼠海马 β位淀粉样蛋白42 和裂解酶1 的影响* 1,2 2 2 1,2 徐 波 徐 静 余 锋 季 浏 摘要 目的：探讨游泳+跑台的混合运动训练对D-半乳糖所致阿尔茨海默病（AD）模型大鼠海马内β位淀粉样蛋白42和裂 解酶1（Aβ42、BACE1）的影响。 方法：将16只雄性SD大鼠随机分为安静注射组(C组)和运动注射组(T组)，所有大鼠连续8周腹腔注射D-gal造阿尔 茨海默病模型大鼠，同时，T组大鼠连续进行8周的游泳和跑台（均每周3次，交替进行）相结合的混合运动训练。运 用Western Blot法检测大鼠海马Aβ42蛋白表达量，运用RT-PCR法检测大鼠海马BACE1mRNA和IGF-1mRNA的表 达量。 结果：①与C组相比，T组大鼠海马内Aβ42蛋白表达量显著减少，具有极显著性差异（P0.01）；②C组比T组大鼠海 马内BACE1 mRNA表达量多，具有显著性差异（P0.05）；③与C组相比，T组大鼠海马内胰岛素源生长因子 （IGF）-1mRNA表达增加，具有极显著性差异（P0.01)。 结论：游泳和跑步混合运动训练能延缓AD的发展进程，这可能与运动训练上调了AD大鼠海马内IGF-1mRNA表达 有关。 关键词 运动训练；D-半乳糖；阿尔茨海默病；海马；β-淀粉样前体蛋白42；β位淀粉样前体蛋白裂解酶1；胰岛素 源生长因子 中图分类号：R493,R749.1 文献标识码：A 文章编号：1001-1242（2012）-02-0111-04 Effects of exercise training on β-amyloid protein 42 and β-amyloid precursor protein cleaving enzyme 1 in hippocampus of D-galactose induced Alzheimers disease model rats/XU Bo ，XU Jing ，YU Feng ，et al.// Chinese Journal of Rehabilitation Medicine, 2012, 27(2): 111—114 Abstract Objective: To study the effects of swimming and treadmill training on hippocampal β-amyloid precursor protein 42(Aβ42)anditscleavingenzyme1(BACE1) inrat modelof D-galactose inducedAlzheimersdisease(AD). Method: Sixteen SD male rats were randomly divided into sedentary injection group (C) and exercise injection group (T). All of the rats were received injection of D-galactose into abdominal cavity continuously for 8 weeks to make AD animal models. Group T were in addition given mixed training (swimming and treadmill training al- ternatively each 3 times per week) for 8 weeks. The levels of hippocampal Aβ42 protein of rats were inspected by Western