L_精氨酸对在体兔肺缺血_再灌注损伤影响的实验研究.pdfVIP

2008 9 25 9 J Clin R es , S ep. 2008, V ol 25, 9 1569 论著 L- - 朱加亮龚伟刘建新王电军金龙玉洪朝姜志斌 ( 中南大学湘雅三医院心胸外科, 湖南长沙410013) [ ]目的探讨L- 精氨酸对兔肺缺血- 灌注损伤的影响方法建立在体兔肺缺血- 灌注模 型, 随机分成三组: A 组为假手术组B 组为缺血- 灌注组C 组为缺血- 灌注+ L- 精氨酸处理组, 每组7 只在 灌注60 m in 行肺组织湿干比W / D 值丙二醛( M DA ) 超氧化物歧化酶( SO D) 含量测定和肺组织病 理学检查结果 灌注60 m in 后C 组肺组织W / D 值M DA 含量较 B 组均明显降低( P 0. 05) , 而 SO DNO 含量较B 组明显升高( P 0 . 05) 肺组织病理学检查示 BC 组兔肺均有部分肺泡结构破坏, 不同 程度的炎症反应, 其中C 组炎症积分显著低于B 组( P 0. 0 1) 结论L- 精氨酸预处理 , 可减轻随后的缺 血 灌注损伤 [ ] 灌注损伤 肺/ 病理学 精氨酸/ 药理学 Effect of L-arginine on Pulmonary Ischemia- reperfusion Injury in Rabbit ZH U Jia-liang , GON G Wei, LI U Jian-xin, et al ( Dep artment of Thoracic and ardiovascular S urgery , Third X iangya H osp ital, entral South University, hangsha 4100 13, H unan ) [Abstract]O bjectiveT o study the eff ect o f L- ar ginine o n lung ischem ia- r eperfu sio n inj ur y in rabbit . M ethods T he ischem ia- rep erfu sion r abbit w as con st ru cted and divided into thr ee g ro up s randomly: g ro up A ( no surg ery) , g ro up B ( ischem ia- rep erfu sion) and g ro up C( L- arg inine + ischem ia- rep erfu sion) . T he w et- dry rate ( W/ D) , M DA content , serum NO co ncentration and the patho log y of lung tissu e w ere m easured after 60m in rep erfu sion . Result sA fter 60m in r eper fusion , W/ D and M DA in g ro up C w ere low er th an in those in gr oup A and B ( P 0. 05) , w hile the concent ratio n of N O and SO D w er e higher th an tho se in g ro up A and B( P 0. 05) . T he inflamm ator y ch anges of pulmo na ry tissu e in gr oup C w as o bvio usly less th an in g roup B ( P 0 . 05) . Conclu sion L- arg inine can prev ent ischem ia- r eper fusio n inj ur y in r abbit lung in vivo. [Key words] r ep erfu sio n inj ur y lung/ P