紫苏子油的化学成分研究_547.docVIP

紫苏子油的化学成分研究 谢先吉 张相宜（浙江大学医学院附属第一医院，杭州310003） 摘要 目的：对超临界CO2萃取的紫苏子油的化学成分进行研究。方法：采用超临界二氧化碳流体萃取法(SFE-CO2)提取；通过气相色谱-质谱联用技术(GC-MS)分析鉴定化合物结构，色谱条件：石英毛细管柱HP－Innowax（30m×0.25mm×0.50μm），进样口温度250℃，程序升温，柱温8O℃，保持5min，以5℃·min-1的速度升至120℃，保持10mim，再以6℃·min-1升温至230℃,保持30min。载气He，进样量为1微升，分流比5。EI7OeV，全离子扫描范围m/z 40～500，以峰面积归一化法计算各成分相对百分含量。结果：分析鉴定了79种化学成分，所鉴定的成分占总流出峰面积的91.59％。结论：为紫苏子油的药效物质基础进一步研究提供科学依据。 关键词：紫苏子油；超临界二氧化碳流体萃取法；气相色谱-质谱联用 Compositional Analysis of Perilla Seed Oil XIE Xian-ji, ZHANG Xiang-yi (The First Affiliated Hospital，College of Medicine，Zhejiang University; Hangzhou 310003; China ) Abstract Objective：Analyze the constituents in supercritical fluid CO2 extraction(SFE-CO2)of perilla seed oil with gas chromatography mass spectrometry．Methods：Perilla seed oil was extracted with SFE-CO2，and structure analyzed by GC-MS．The GC-MS analysis with a quartz capillary column HP-Innowax (30m×250um×0.50um film thickness)was used．The inlet temperature was maintained at 250℃．The column oven was begun at 80℃，held for 5 min．Then programmed from 80 to 120℃ at 5℃·min-1，held for 10 min，programmed 230℃ at 6℃·min-1，held for 30 min．Helium was used as the carrier gas．The inlet volum was 1 μl, diffluence rate was 1:5. The mass spectrometry conditions were as follows：ionization energy，70eV；ion source temperature，230℃．The mass selective detectorwas operated in the TIC mode(m/z 40-500)．Results：By the analysis of the SFE-CO2 extractions of perilla seed oil 79 peaks were separated and identified，the compounds were quantitatively determined by normalization method．The identified compounds represent 91.59％ of total GC peak areas．Conclusion：The results will provide important foundation for understanding the constituents and further exploitation of perilla seed oil． Key words：perilla seed oil；SFE-CO2 extraction；GC-MS Perilla frutescens[L] Britt.）的干燥成熟果实，有降气消痰，平喘，润肠之功效，临床用于痰壅气逆，咳嗽气喘，肠燥便秘。收载于中华人民共和国药典2010版一部。现代药理学研究表明：紫苏子含有两大类抗过敏、炎症物质，即脂肪酸类成分和多元酚类成分[1]。它们均属于过敏介质拮抗剂类。紫苏子油含有高达70％以上，抑制亚油酸向花生四烯酸转化，从而降低体内花生四烯酸水平