[医学]生物钟蛋白翻译后的化学修饰.pdfVIP

生物钟蛋白翻译后的 化学修饰 Qun He Department of Microbiology Immunology, College of Biological Sciences, CAU Circadian Rhythms • Endogenous rhythm which persists under constant conditions • Period of approximately 24 hours • Entrainable (reset) by environmental cues such as light and temperature • Temperature compensated - period stays about the same at different temperatures Circadian Oscillators Positive (-) Element(s) (+) Negative Element(s) clock gene (s) Negative elements in circadian feedback loops: Synechococcus: kaiC Neurospora: FREQUENCY Drosophila: PERIOD and TIMELESS Mouse: mCRYs and mPERs Positive elements in circadian feedback loops Synechococcus: kaiA PAS domain containing Neurospora: WHITE COLLAR-1 and WC-2 transcription factors Drosophila: dCLOCK and CYCLE Mouse: CLOCK, NPAS2 and BMAL1 Neurospora crassa (bread mold) • Non-pathogenic • Growth, transfer, stock and manipulation of the cultures very easy to handle • The complete genome sequence available • Exhibits one of the most easily assayed natural circadian rhythms