Localization of the PE methylation pathway and SR BI to the…甲基化途径和定位于.pdfVIP

Localization of the PE methylation pathway and SR-BI to the canalicular membrane: evidence for apical PC biosynthesis that may promote biliary excretion of phospholipid and cholesterol 1, † § Ephraim Sehayek, * Rong Wang, Jennie G. Ono,* Vadim S. Zinchuk, Elizabeth M. Duncan,* Sarah Shefer,** Dennis E. Vance,†† Meenakshisundaram Ananthanarayanan,§§ Brian T. Chait,† and Jan L. Breslow* Laboratory of Biochemical Genetics and Metabolism,* and Laboratory of Mass Spectrometry and Gaseous † Ion Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10021; Department of Anatomy § and Cell Biology, Kochi Medical School, Kochi, Japan; Department of Medicine and Liver Center,** University of Medicine and Dentistry of New Jersey, Newark, NJ 07103; CIHR Group on Molecular and Cell Biology of Lipids and Department of Biochemistry,†† University of Alberta, Edmonton, Alberta, T6G 2S2 Canada; and Department of Pediatrics,§§ Laboratory of Developmental and Molecular Hepatology, The Mount Sinai Medical Center, New York, NY 10029 Abstract To better understand the regulation of biliary E. M. Duncan, S. Shefer, D. E. Vance, M. Ananthanarayanan, phospholipid and cholesterol excretion, canalicular mem- B. T. Chait, and J. L. Breslow. Localization of the PE methyl- branes were isolated from the livers of C57BL/6J mice and ation pathway and SR-BI to the canalicular membrane: evi- abundant protein