Long Term Detection of Human Adipose Derived Mesenchymal Stem…人类脂肪来源间充质干细胞的长期检测.pdfVIP

ARTHRITIS RHEUMATISM Vol. 65, No. 7, July 2013, pp 1786–1794 DOI 10.1002/art.37960 © 2013, American College of Rheumatology Long-Term Detection of Human Adipose-Derived Mesenchymal Stem Cells After Intraarticular Injection in SCID Mice Karine Toupet,1 Marie Maumus,1 Julie-Anne Peyrafitte,2 Philippe Bourin,2 Peter L. E. M. van Lent,3 4 5 6 Rosanna Ferreira, Be´atrice Orsetti, Nelly Pirot, Louis Casteilla,7 Christian Jorgensen,8 and Danie`le Noe¨l1 Objective. Mesenchymal stem cells (MSCs) rep- tration of 106 human AD-MSCs. Several tissues were resent a promising tool for cell therapy for several recovered at different time points and processed for disorders, among them the osteoarticular diseases. For histologic assessment or real-time polymerase chain such clinical applications, intraarticular (IA) injection reaction (PCR) analysis. A highly sensitive assay was of MSCs may be favored for higher levels of safety and used to monitor the distribution of AD-MSCs, based on targeting of specific joints. Although the safety of intra- amplification of human-specific Alu sequences