氨基酸分析EP7.0.pdfVIP

2.2.56. Amino acid analysis EUROPEAN PHARMACOPOEIA 7.0 The numerical comparison of the peak retention times and and peptides are macromolecules consisting of covalently peak areas or peak heights can be done for a selected group of bonded amino acid residues organised as a linear polymer. The relevant peaks that have been correctly identified in the peptide sequence of the amino acids in a protein or peptide determines maps. Peak areas can be calculated using 1 peak showing the properties of the molecule. Proteins are considered large relatively small variation as an internal reference, keeping in molecules that commonly exist as folded structures with a mind that peak area integration is sensitive to baseline variation specific conformation, while peptides are smaller and may and likely to introduce error in the analysis. Alternatively, the consist of only a few amino acids. Amino acid analysis can be percentage of each peptide peak height relative to the sum of all used to quantify proteins and peptides, to determine the identity peak heights can be calculated for the sample under test. The of proteins or peptides based on their amino acid composition, percentage is then compared to that of the corresponding peak to support protein and peptide structure analysis, to evaluate of the reference substance. The possibility of auto-hydrolysis of fragmentation strategies for peptide mapping, and to detect trypsin is monitored by producing a blank peptide map, that atypical amino acids that might be present in a protein or is, the peptide map obtained when a blank solution is treated peptide. It is necessary to hydrolyse a protein/peptide to its with trypsin.