Application of hyperthermophiles and their enzymes推荐.pdfVIP

Available online at Application of hyperthermophiles and their enzymes Haruyuki Atomi1,2, Takaaki Sato1,2 and Tamotsu Kanai1,2 Enzymes from hyperthermophiles display extreme explored. The accumulation of (meta)genome sequences (thermo)stability and a wide range of enzymes have been in the databases and the tools to search for desired examined to explore their potential for various biotechnological sequences, along with the ease to synthesize genes for processes. In addition, recent years have witnessed the expression in conventional host cells provides us with the development of genetic systems in a number of means to examine a large number of gene products. This hyperthermophilic archaea. This has provided the means to approach on one hand will not lead to the discovery of initiate cell engineering studies in these organisms. Biofuel structurally novel enzymes, but allows a rapid search for production is now an important topic in microbial enzymes that exhibit differences in selectivity towards biotechnology, and the hydrogen producing capabilities of a particular substrate or stability towards a certain (hyper)thermophiles, as well as their thermostable parameter such as temperature, pH or solvents. Various hydrogenases, are now attracting much attention. methods for high throughput activity and gene expression Addresses screening have also been developed [2–5], and can be 1 Department of Synthetic Chemistry and Biological Chemistry, Graduate used for both enzyme engineering and structurally novel School of Engineering, Kyoto University, Kyoto 615-8510, Japan enzyme discovery. 2 JST, CREST, Sanbancho, Chiyoda-ku, Tokyo 102-0075,