核酸特性、结构和功能（英文版）.pptVIP

19 Are Nucleosomes “Positioned?” 1. Sequence at Unique Position 2. Micrococcal Nuclease Digest 3. Restriction Digest 4. Southern Blot Discrete Band Positioning Smear Random Orientation 17 Interference Footprinting DNA + MMS + Saturating [Protein] Gel Shift 14 Cleave backbone @ modified bases “Chromatin is the last refuge of scoundrels” A Mitotic Human Chromosome DNA length: 4.6 x 107 bp = 1.5 cm Chromosome length: 2 μm Compaction ratio = 8000 1 Chromatin DNA Compaction DNA Accessibility Chromatin Remodeling Machines NIH Basic Unit of Chromatin Structure