discovery of lung squamous carcinoma biomarkers by profiling the plasma peptide with lcmsms：（发现肺鳞状细胞癌生物标志物分析等离子体与lcmsms肽）.pdfVIP

Chinese Chemical Letters 28 (2017) 1093–1098 Contents lists available at ScienceDirect Chinese Chemical Letters journal homepa ge: /locate/cclet Original article Discovery of lung squamous carcinoma biomarkers by profiling the plasma peptide with LC/MS/MS Yu Liu, Xiao-Hong Xun, Jian-Ming Yi, Yang Xiang, Jie Hua* College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414006, China A R T I C L E I N F O A B S T R A C T Article history: Received 31 July 2016 Biomarkers can be used for the screening and clinical diagnosis of cancer, and peptidomics approach has Received in revised form 14 October 2016 been proven successful in the research of biomarkers. To develop better peptidomic technologies for fast, Accepted 28 October 2016 accurate, and reliable detection of peptides biomarkers for lung cancer, we have improved the procedures Available online 27 November 2016 of blood collection to minimize the degradation of the blood proteins and optimize the extraction of peptidome peptides from plasma samples based on acetonitrile precipitation associated with size Keywords: exclusion chromatography (SEC). Studies show that squamous cell carcinomas are found to express Lung cancer CAGE1, SPAT9 and TEX28 genes at significantly higher rates, and the results suggest that as tumors Lung squamous carcinoma progress, the level of CAGE1, SPAT9 and TEX