基于脲酶催化和酸碱指示剂变色原理的高脲乳快速检测方法的建立.PDF

222 2011, Vol. 32, No. 10 食品科学 ※分析检测 杨水云，郑 潇，韩 禹，隆永秋，刘永青 (西安交通大学生命科学与技术学院，线粒体医学研究所，生物医学信息工程教育部重点实验室，陕西 西安 710049) 针对市场上存在的高脲乳(掺假和病理性) 问题，建立区别于正常生理浓度范围的快速检测方法。检测方法 依据尿素被酶解导致的pH 值升高幅度与其浓度呈正相关这一机理，对牛乳起始pH 值进行优化调整为 6.8 时，选择 溴百里酚蓝作为指示剂，可以使乳脲含量达40mg/100mL 生理上限卡切值以上时变色，从而区别检出高脲问题乳。 研究表明，依此组装的高脲乳检测试剂盒，可用于生产实践。 尿素掺假乳；病理性高脲乳；检测试纸；检测试剂盒；脲酶；乳脲氮 Rapid Detection Method for High-Urea Milk Based on Urease Catalysis YANG Shui-yun ，ZHENG Xiao ，HAN Yu ，LONG Yong-qiu ，LIU Yong-qing (Key Laboratory of Biomedical Information Engineering, Ministry of Education, Institute of Mitochondrial Biology and Medicine, School of Life Science and Technology, Xi’an Jiaotong University, Xi ’an 710049, China) Abstract：The objective of this study is to establish a rapid detection method for milk with urea content exceeding physiological range. Based on the fact that pH increase could result from urea enzymolysis positively correlated with urea concentration. Bromothymol blue was chosen as the acid-base indicator to determine high-urea milk. The indicator can develop color from yellow-brown to green-blue when urea content was higher than 40 mg/100 mL. On the basis of our present study, the developed reagent kit for the determination of high-urea milk is useful for practical application. Key words ：adulterated milk ；pathological high-urea milk ；test paper ；milk-urea reagent kit ；urease ；milk urea nitrogen 中图分类号：TS201.6 文献标识码：A 文章编号：1002-6630(2011)10-0222-05 尿素，别名脲，碳酰胺，是含氮化合物在哺乳动 料乳中尿素的正常生理浓度通常情况下应介于 3 0 ～ 物肝脏内经由尿素循环产生，之后融入血液，最后通 36mg/100mL[4]之间，若超过40mg/100mL 则视为高脲乳或 过肾脏由尿排出体外。在体内，血尿素可以扩散进入 不合格牛乳[5] 。人体血脲正常参考范围为 2.8 ～8.2mmol/L 动物乳中，因此是