(精编)【医学教学课件】分子生物学技术 Techniques of Molecular Biology教学课件.ppt

* Assemble the contigs from 1kb plasmid shotgun sequence. (50 kb-200 kb) Assemble the contigs to large scaffold by sequencing both ends of 5 kb plasmids. (500 kb) Assemble the larger scaffolds (1 Mb) by sequencing the end of the BAC library. Assembly flowchart * Genome-wide analysis The purpose of this analysis is to predict the protein coding genes (蛋白质编码基因) and other functional sequences (其他功能序列) in the genome. * For the genomes of bacteria and simple eukaryotes： Finding protein coding genes = Identification of ORF (open-reading frames). straightforward; fairly effective; but not all ORF