肾性骨病模型大鼠皮质骨骨量及结构变化的研究.DOCVIP

肾性骨病模型大鼠皮质骨骨量及结构变化的研究 陈珺1，钟雁1，孟彦2，张豪1，1，陆幸研1，李敏1，左力2*，李青南1* 广东药学院生命科学与生物制药学院新药功能研究中心，广州，510006北京大学第一医院肾内科北京大学肾脏病研究所教育部肾脏病重点实验室教育部慢性肾脏病的预防与治疗重点实验室，北京，100034 摘要：目的 腺嘌呤高磷模型骨矿代谢。方法3月龄雄性SD大鼠只，随机分高磷饮食给予腺嘌呤高磷饮食，高磷饮食体内双荧光标记取左侧胫骨不脱钙包埋织形态计量学分析。结果 与C组相比组组矿化沉积率、明显增加骨矿化明显结论骨吸收骨吸收大于骨形成明显的骨高转换。 关键词腺嘌呤；高磷饮食；高转；肾性骨病模型 中图分类号： 文献标识码： 文章编号： a Mouse Model of Renal Osteodystrophy Caused by High Phosphorus Diet with Adenine-containing CHEN Jun1, ZHONG Yan1, MENG Yan2, ZHANG Hao1, YANG Guo-zhu, LU Xing-yan, LI Min, ZUO Li2, LI Qing-nan1* 1. the Center for New Drug Function Research, School of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China. 2. Renal Division, Peking University First Hospital, Beijing, China; Peking University Institute of Nephrology, Beijing, China; Key Laboratory of Renal Disease, Ministry of Health, Beijing, China; Key Laboratory of Chronic Kidney Disease Prevention and Treatment (Peking University), Ministry of Education, Beijing, China. Abstract: Objective: To observe the cortical bone changes in the renal osteodystrophy model by feeding an adenine and high phosphorus diet to rats, and discuss the effects on bone mineral metabolism. Methods: 37 cases of male SD rats were randomly divided into control group(C group were fed on high phosphorus diet for all 6 weeks ) and model group(M group were fed an adenine and high phosphorus diet for 4 weeks followed a high phosphorus diet without adenine for 2weeks). All rats were injected subcutaneously with calcein (10 mg/kg) at 13, 12, 3, and 2 days before sacrifice for fluorescent labeling, The left tibia shaft was taken for bone histomorphometry after uncalcified embedded. Results: Bone histomorphometry static parameters showed that total tissue area, percent cortical area and cortical width were significantly decreased in M group, while the percent marrow area was increased, comparing with those in C group. Erosion holes were found in cortical bone. Dynamic paramet