从磷脂酰爬行酶到白血病细胞分化.pptVIP

FROM Phospholipid Scramblase 1 (PLSCR1) TO Leukemic Cell Differentiation从磷脂酰爬行酶到白血病细胞分化 Health Science Center，SIBS Zhao Ke-Wen In mice Normal hemostasis but defective hematopoietic response in plscr1-/-mice ; MmTRA1a, a truncated form of murine PLSCR1 was identified in a monocytic leukemia cell line TO Share with you acknowledgements SIBS, AC Guo-Qiang Chen; Qian Zhao; Xi Li; Dong Li; Ying Huang; Ci-Xiang Zhou; Jing Zhang; Zhen-Gang Peng; Shi-Hua Liao Mei-Yi Zhou; Meng Zhao Conclusion1:PLSCR1 can be up-regulated by activation of PKC? PLSCR1 Leukemic cell differentiation ? Inhibition of PLSCR1 expression by siRNA partially blocks ATRA- and PMA-induced differentiation c p r c p r c p r P2 P5 ?-actin empty PLSCR1 A control PMA ATRA cell number CD11b 11.6% 3.0% 3.9% 71.2% 63.8% 49.6% 87.9% 62.7% 48.6% B P5 P2 empty 10-6 M ATRA – + empty P5 iRNA C c: control p: PMA r: ATRA Conclusion2: PLSCR1 involves in leukemic cell differentiation 2004-4-28 submission to BLOOD 2004-5-26 BLOOD response : ‘I am happy to report that the paper was favorably received and,provided an appropriate revision is prepared, can be acceptedfor publication in Blood.’ ‘interesting, convincing ‘ (Blood. 2004;104:3731-3738) BLOOD, 1 DECEMBER 2004 VOLUME 104, NUMBER 12 团队精神 充分的准备 主动地交流 实干+巧干 规范实验 +归纳总结 正确地对待挫折和失败 Linda B. Buck, Ph.D. ????????????????????????? * 第一作者论坛 恶性肿瘤是威胁人类健康的主要杀手。 在我国，白血病占恶性肿瘤的第7位 细胞分化 造血干细胞 成熟血细胞 白血病 （Leukemia） Specific translocation t(15;17) Chromosome 17 RARa: Retinoic acid receptor-alpha Chromosome 15 PML: promyelocytic leukemia 急性早幼粒细胞性白血病（APL） -PML RARa Acute promyelocytic Leukemia (APL) PML PML RARa RARa PML PML RARa RARa T(15;17) As2O3 ATRA All-trans-retinol All-trans-retinoic acid OH COOH 13-cis-Retinoic acid COOH COOH OH CH2OH 9-cis-Retinoic acid 14-Hydroxy-4, 14-retro-retinol (Adapted from Warrell et al, N Eng J Med, 1993) APL is curable Di