SRB的筛选.docVIP

一株硫酸盐还原菌的分离筛选及生理特性的初探 学生 指导老师 乐以成 摘要：硫酸盐还原菌利用其它细菌的代谢终产物作为自己的能源而生长，其本身又是还原活动的参与者。而硫酸盐有机废水生物处理技术一直是人们关注的热点，这种废水处理的关键是如何在有效去除COD的同时，高效的去除SO42-。硫酸盐还原菌在一定的条件下可以有效的将SO42-还原从而从废水中去除，并在该过程中起主要作用。为了更深入的研究其过程机理，比较好的做法是把在那种在数量上占优势，并且起主要功能的种群分离出来，对它们进行单独研究。本文利用厌氧装置和滚管技术，于广东化工集团硫酸厂废水污泥中分离和筛选了一株硫酸盐还原菌，命名为SRB6。并对其生理生化指标进行了测定。菌体为短杆状或弧状，0.5—0.6μm ，革兰氏染色阴性，不产芽孢。有Fe2+时菌落为黑色，直径为2mm，无Fe2+时固体培养基上的菌落为灰白色，直径1.5mm。利用乳酸盐作为最佳碳源。最适生长盐浓度为0.5%（W / V ），最适生长温度为35℃，最适pH值为7.0。根据形态和生理生化特征，该菌为脱硫弧菌属。但与该属已报道的硫酸盐还原菌在碳源利用、最适生长温度、最适盐浓度及其它一些生理特征方面均有不同。 关键词：硫酸盐还原菌、厌氧培养、Hungate试管 ISOLATION AND PHYSIOLOGY OF A SULFATE-REDUCING BACTERIUM Student Teacher Abstract ： Sulfate-reducing bacteria use the final production of other bacteria as the energy source , and it also participate in the reducing action . The biological treating technology is always the focus of researchers in the world . The key of this technology is to remove COD and SO42- simultaneously and effectively . Sulfate – reducing bacteria can reduce SO42- to H2S as to rtemove SO42- . To study physiological mechanism in depth , we isolating main functional group , SRB can simplify this research . Preponder ant population of SRB were isolated . Using anaerobic set and Hungate tube technic , a sulfate-reducing bacteria was isolated from the wastewater of Chemical Industry Corporation Vitriol Factory . And we mensurate its physiology guide. The strain is gram negative and not spore forming . Its short rod or arc , and single cell is about 0.5?.6 μm wide and 1.7—2.0 μm long . Colonies from roll tubes without Fe2+ are grey white and about 1.5mm in diameter . When media contain , Fe2+ the colonies are black and about 2mm in diameter . It utilize lactate as carbon source . For its growth , the optimum NaCl concentration range is 0.5%（W/V) , temperate 36℃ and pH