酶底物法快速检测食品中的大肠埃希氏菌-食品安全质量检测学报.PDFVIP

第8 卷 第 10 期 食品安全质量检测学报 Vol. 8 No. 10 2017 年10 月 Journal of Food Safety and Quality Oct. , 2017 酶底物法快速检测食品中的大肠埃希氏菌 蒋玉佳, 颜 军, 谢超华, 魏茂琪, 杨晓蓉, 侯 敏* (昆明市疾病预防控制中心, 昆明 650228) 摘 要: 目的 将水质检验的酶底物法用于食品检验, 探索酶底物法是否能快速检测食品中的大肠埃希氏 菌。方法 测定食品样品, 比较酶底物法与传统鉴定法结果的一致性。结果 传统方法检出24 份阳性和8 份 2 2 阴性, 酶底物法检出25 份阳性和7 份阴性。实验结果进行X 检验, 结果无显著性差异(X =0.087, P ＞0.05) 。 32 份样品MPN 值结果一致, 8 份样品MPN 值有差异, 其中7 份样品酶底物法的MPN 值大于传统法。结论 酶 底物法可用于食品中大肠埃希氏菌的快速检测, 具有快捷简便、特异性高的优势。 关键词: 食品; 大肠埃希氏菌; 酶底物法 Rapid detection of Escherichia coli in food by enzyme substrate method * JIANG Yu-Jia, YAN Jun, XIE Chao-Hua, WEI Mao-Qi, YANG Xiao-Rong, HOU Min (Kunming City Center for Disease Control and Prevention , Kunming 650228, China) ABSTRACT: Objective To introduce the enzyme substrate method for water quality testing to test food, in order to explore whether the enzyme substrate method can quickly detect Escherichia coli in food. Methods The consistency of enzyme substrate method and traditional identification method were compared by testing food samples. Results Totally 24 positive samples and 8 negative samples were detected by traditional dentification method, and 25 positive samples and 7 negative samples were detected by enzyme substrate method. The experimental results had 2 2 no significant difference checked b