血清结肠癌特异性抗原-2检测在结直肠癌诊断中的价值.docVIP

血清结肠癌特异性抗原-2检测在结直肠癌诊断中的价值 薛刚1，周均2，曹永宽2，王晓娟1，杨勇1，杨朝春1 [摘要] 目的 评估血清结肠癌特异性抗原-2（CCSA-2）检测在结直肠癌诊断中的价值。 方法 收集105例住院患者（含结直肠癌、胃癌、腹股沟疝、急性阑尾炎以及乳腺癌）和20名健康志愿者术前3天的血液样本各3ml（其中急性阑尾炎患者在急诊手术前采血）, 用酶联免疫吸附法测定其中CCSA-2的含量。 结果 CCSA-2在结直肠癌患者血清中的含量明显高于其他患者及健康志愿者(F=685.91,P0.01)。通过受试者特征曲线分析，当以73.96ng/ml 为截断值时，血清CCSA-2用于诊断结直肠癌的敏感性和特异性均能达到100%（95%可信区间：100-100%）。此外，血清CCSA-2用于诊断结直肠癌的敏感性明显高于传统标志物CEA和CA19-9 (P0.01)。结直肠癌患者血清中CCSA-2的含量与肿瘤分期、分级以及 多药耐药相关蛋白、P糖蛋白、拓扑异构酶erum CCSA-2 detection in diagnosis of colorectal cancer XUE Gang, ZhOU Jun, CAO Yong-kuan, WANG Xiao-juan, YANG Yong, YANG Chao-chun. Department of Breast and Thyroid Surgery, Chengdu Military General Hospital, Chengdu 610083, P.R. China Corresponding author: XUE Gang, E-mail: kpardan@163.com [Abstract] Objective: To evaluate the value of serum colon cancer-specific antigen-2(CCSA-2) detection in diagnosis of colorectal cancer(CRC).Methods: Using an ELISA method, the CCSA-2 protein was measured in the serum from 105 various patient populations(including CRC patients, gastric cancer patients, inguinal hernia patients, acute appendicitis patients and breast cancer patients) and 20 health donors on day 3 before surgery(the serum sample from acute appendicitis patients were collected before emergency surgery). Results: The serum CCSA-2 content in CRC patients was significantly higher than which in other patients and healthy individuals(F=685.91,P0.01). Serum CCSA-2 at a cutoff 73.96ng/mL had a sensitivity of 100%( confidence interval, 95%CI, 100–100%) and a specificity of 100%( 95%CI, 100–100%) in separating CRC populations from all other individuals using receiver operator characteristic(ROC) analyses . As compared to CEA and CA19-9, the CCSA-2 assay was significantly more sensitive than CEA and CA19-9 assay in CRC detection(P0.01). There was no relation between CCSA-2 content and tumor stage, grade as well as MRP, P-gp and TOPⅡexpression. Conclusion: Serum CCSA-2 may be a useful biomarker in diagnosis of CRC, it may only related to tumo