蛋内接种伏马镰孢毒素对鸭胚发育之影响郑永祥1张铭煌2赵智宇3摘要.pdf

蛋內接種伏馬鐮孢毒素對鴨胚發育之影響 1 2 3 鄭永祥 張銘煌 趙智宇 1.國立宜蘭技術學院畜產學系 (通訊作者) 2.國立嘉義技術學院獸醫學系 3.國立宜蘭技術學院畜產學系 摘要 本研究之目的旨在探討，不同濃度伏馬鐮孢毒素(Fumonisin B1 ；FB1蛋內) 接種後對鴨胚發育之影響。試驗使用200 枚菜鴨受精蛋，逢機分配於5 組，每 處理二重複，每重複 10顆蛋，分別於蛋內接種 0 、20 、40 、80或 160 μg/egg 純化之FB1 ；接種用FB1 分別溶於160 μl 二次蒸餾水並經過濾滅菌 稀釋液( ) ， 而對照組者僅接種稀釋液。試驗一，蛋於孵化第1日接種，試驗期間記錄死亡 率及接種後14日時之體測性狀。試驗二則在孵化第 14日接種，記錄死亡率及 接種後7日時之體測性狀。試驗結果顯示死亡率會隨著時間及劑量增加，試驗 一中以20 μg 、40 μg 、80 μg或 160 μg FB1 接種者，其死亡率分別為10﹪、 20﹪、20﹪及100﹪；而在第14日接種者，除 40及 160 μg FB1有 10﹪死亡 率，其於均無死亡。在體測性狀方面，僅試驗一中20 μg 接種組有顯著較長之 頸長。無論是第 1日或第 14日 40 μg 以上接種者，鴨胚之肝、腎及心臟均可觀 察到組織病理學變化。 關鍵詞鴨胚、伏馬鐮孢毒素、蛋內接種: Effect of Fumonisin Inoculation on Duck Embryo Development in ovo Yeong-Hsiang Cheng1 Ming-Haung Chang2 Chi-Yu Chao3 1. Department of Animal Science, National I-Lan Institute of Technology(Corresponding Author) 2. Department of Animal Science, National I-Lan Institute of Technology 3. Department of Animal Science, National I-Lan Institute of Technology Abstract This study was design to investigate the different concentration of Fumonisin B1 (FB1) on duck embryo development after in ovo inoculation. There were 200 fertile eggs of Tsaiya being used, and alloted randomly into five treatment groups as follows 0, 20, 40, 80 or 160 μg/egg. FB1 was dissolved in 160 μl of double-distillated water and sterilization by millipore filtration, and control group was inoculated with sterilized water only. In experiment 1, fertile eggs were inoculated at day one , the mortality and the 14th day old body performance were recorded. In experiment 2, eggs were inoculated at 14th day old, and the mortality