Molecular Strategies of Combinatorial Control and Gain in Gene 组合控制和增益基因的分子策略.ppt

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2018-04-17 发布于江西
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Molecular Strategies of Combinatorial Control and Gain in Gene 组合控制和增益基因的分子策略.ppt

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Gene networks and electronic circuits This talk ?focus on individual node of gene network ?quantitative examination of bacteria gene regulation ?connection between molecular and functional aspects Transcriptional regulation in bacteria activation of transcription factors (TF) binding of TF(s) to DNA targets (~10 bp) enhance transcription by recruitingRNA polymerase (RNAp) to its target (“promoter”) Complex transcriptional control Molecular basis of complex transcriptional control? specific, complex protein-protein interaction Theory of Combinatorial Transcription Control take regulated recruitment as starting point include thermodynamics of TF/DNA/RNAP interaction implement control functions of increasing complexity using bacterial transcriptional mechanisms Illustration of combinatorial control: Illustration of combinatorial control: Illustration of combinatorial control: Illustration of combinatorial control: More complex control functions, e.g., XOR ? More complex control functions, e.g., XOR ? More complex control functions, e.g., XOR ? Quantitative modeling: Simple transcriptional control Simple transcriptional control Simple transcriptional control Simple transcriptional control Full Plac promoter more complicated Alternative realizations of “AND gate” Quantitative design for OR Quantitative design for XOR Complex transcriptional control Signal Amplification in Genetic Circuits Self-activating gene with dimeric TF Self-activating gene with dimeric TF Self-activating gene with dimeric TF dimeric TF with nonlinear degradation dimeric TF with nonlinear degradation dimeric TF with nonlinear degradation dimeric TF with nonlinear degradation Nonlinear degradation of oligomers This talk ?focus on individual node of gene network ?quantitative examination of bacteria gene regulation ?connection between molecular and functional aspects Acknowledgement theory Nicolas Buchler (circuits and signal amplification) Ulrich Gerland (combinatorial control) Weiqun Peng (mo