Dr Daniel Pentek presentation：丹尼尔博士pentek演示.pptVIP

Evolving Role of Mass Spectrometry in Bioanalytical Analysis Daniel Pentek February 1, 2007 -UCONN Bioanalytical Chem 395 Topics to be covered: Quick Review- MS Market Technologies Basic ionization methods Electrospray, APCI, APPI Interfaces Orifice, capillary MS Analyzers Performance Criteria Strengths weaknesses for bioanalytical analyses Quadrupole (single triple quads) Ion traps (3-D and 2-D) Time of flight (linear, reflectron, MALDI) Hybrids (Quad-TOF) and others… FTMS (ICRs and orbitraps) What is a mass spectrometer? An instrument that separates molecules or atoms (e.g., ICP/MS) based on their mass/charge ratio (m/z). In order to control them, you need to put a charge on them. In order to separate them, you need an “analyzer” of some type… LC/MS Historical Perspective Industry was desperate for a decent, rugged LC/MS interface. GC/MS required derivatization, etc. Not applicable to most biomolecules (MW, etc.) API ionization ALONG WITH new interface designs provided the solution. Now, all interfaces are differentially pumped. Pumping of interfaces was critical Orifice – skimmer (nozzle) designs Heated (or cold) capillary designs LC/MS is big business now! Analytical Instruments Technology Segments… SDI data on analytical instrument technology shows mass spectrometry is a $2B market in 2006, and predicts… Distribution of Mass Spectrometry Techniques* LC/MS(/MS) is a Marriage of Liquid Chromatography and Mass Spectrometry Marriage (like any other close relationship) requires: COMPROMISE! LC Person: “MS is just another detector” MS Person: “LC is just an inlet” What’s good for LC may not be good for MS and vice versa. LC was around a long time before they figured out how to interface it to an MS. LC/MS Instrument Basics:Single MS- Primarily used as a detector Today’s LC/MS Ionization Methods All Done at Atmospheric Pressure On-line techniques: Electrospray (ESI) - Fenn @ Yale ~1984 Shared Nobel Prize in 2002 for this work with K. Tana