冬1-Clinic_Genetics-2-2015.pptVIP

Nested PCR Outter primer Inner primer PCR 3’- -3’ 5’- -5’ 3’- 5’- -3’ -5’ Outter primer PCR Inner primer Duplex PCR of DMD 17, 19, 44, 48 exons and SRY gene from single blastomere -3’ -5’ 3’- 5’- 2. Analyses of isolated (amplified) DNA Direct or after the treatment with restricted endonuclease Southern Blot, Agarose gel Heteroduplexing, SSCP DGGE, Sequencing Comparative genome hybridization Micro-array, DHPLC Blastomere 1 Blastomere