纳米生物学及其应用.pptVIP

Fig. 30. Flow chamber setup for hybridization and visualization of fluorescence signals on the nanoarray. Optical microscope image of an optical fiber-bundle based nanoarray containing individual 300 nmdiameter fibers; (ii) magnified SEM image of a region of the array containing nanobeads; (iii) fluorescent nanobeads in the nanoarray. Biosensors and Bioelectronics 24 (2009) 2488–2493 Fig. 31. Schematic of hybridization assays on the fiber nanoarrays. Biosensors and Bioelectronics 24 (2009) 2488–2493 Early diagnosis for low-stage cancer 1)Identification of effective tumor markers by genomic and proteinomics 2)In situ quantitative profiling of multiple markers by multicolor quantum dots doped nanobeads (nano barcode) Multimodal imaging: NIR Optical imaging +MRI 1)Development of novel imaging probe with non-toxic elements 2)Imaging single cells and single molecules, probe the mechanism of tumor metastasis Targeted therapeutics 1)Synthesis of multifunctional nanoparticles (self-tracking and targeted drug/siRNA delivery), aptamers, peptide 2)Rationally design biodegradable nanoparticles for targeted therapeutics * * * * * * * (b) Fluorescence spectra of mouse skin and QDs with UV excitation. Due to the large Stokes shift of QDs, their fluorescence signals can be shifted to a spectral region where the autofluorescence is reduced. See text for further discussion. * * * * 亓立峰 浙江加州国际纳米技术研究院 前言：纳米微粒概述 Part I: 纳米药物与靶向设计 Part II: 肿瘤纳米分子影像与实时治疗 Part III: 纳米探针 前景展望 What is Nano? Quantum dots, magnetic NP Polymer NP Fig1. A schemetic of nanoscale material Phycoerythrin 前言 某种物质或元素纳米级别粒子: nano Zinic; SiO2， nano gold, CdSe/ZnS, Fe2O3, Polymer NP Degradable nanosphere nanocapsule Polymeric matrix Polymeric membrane Oily or aqueous core 前言 化学法： (1)沉淀法/透析法 把沉淀剂加入到盐溶液中反应后，（将沉淀热处理）得到纳米材料。其特点简单易行，但纯度低，颗粒半径大，聚合体纳米粒子、氧化物。 (2) 微乳液法/乳液扩散法 两种互不相溶的溶剂在表面活性剂的作用下形成乳液，在微泡中经成核、聚结、团聚、（热处理）后得纳米粒子。其特点粒子的单分散和界面性好，聚合体纳米粒子、Ⅱ～Ⅵ族半导体纳米粒子多用此法制备 纳米微粒制备方法 前言 纳米粒子表征方法 测 量 方 法 测 量 功 能 适用的尺寸范围 使用