5羟色胺2a2c受体激动剂doi改善糖尿病大鼠排尿障碍的实验分析 word格式.docxVIP

中文摘要5-羟色胺 2A/2C 受体激动剂 DOI 改善糖尿病大鼠排尿障碍的实验研究残余尿量，从而提高排尿效率，且效应随 DOI 剂量增加而增加。同时 DOI 能增强糖尿病大鼠排尿时 CMGs 中的高频振荡波（HFOs）持续时 间，引起糖尿病大鼠排尿时尿道外括约肌强直收缩过程中阶段性松弛时 间增加。酮色林(0.1 mg/kg)静推后可以逆转 DOI 的效应。对照组大鼠用 药后，大鼠膀胱容量、排尿量下降，HFOs 持续时间下降，而排尿效率在 用药前后的变化无统计学意义，即 DOI 对正常大鼠排尿时 CMGs 的作用 微弱。结论: 建立的糖尿病大鼠模型效果稳定，操作简单，可重复性好。 5-HT2A/2C 受体激动剂 DOI 可以剂量依赖性地增强糖尿病大鼠排尿时尿道 外括约肌的高频舒张和收缩功能，部分恢复糖尿病大鼠排尿时尿道外括 约肌的协调性松弛，从而降低膀胱容量，增加排尿量，减少残余尿量， 提高排尿效率，改善排尿功能障碍。因此 5-HT2A 受体激动剂可能为糖 尿病病人排尿功能障碍提供新的药物治疗。关键词：5-羟色胺受体，糖尿病大鼠，尿道外括约肌，排尿。作者：涂红坚 指导老师：谷宝军5-羟色胺 2A/2C 受体激动剂 DOI 改善糖尿病大鼠排尿障碍的实验研究英文摘要5-HT2A/2C Receptor Agonist DOI Improves Voiding Efficiency in the Diabetic RatAbstractAims: This study to establish the steady and easily replicated models of Type I diabetes mellitus in rats and examine the effects of the 5-HT2A/2C receptor agonist DOI on micturition in the Diabetic Rat.Methods: Female Sprague-Dawley rats (n=16) were divided into two groups: type I diabetes mellitus (DM) rats (n=8)and age-matched controls(n=8). DM was induced by an intraperitoneal injection of streptozotocin (STZ, 65 mg/kg) after 12h of fasting and detailed cystometrogram(CMG) studies was performed 8 weeks post-injection with 10% urethane anesthesia in all rats. The capacity, residual volume, basal bladder pressure,peak bladder pressure, micturition volume and so on were measured. The selective 5-HT2A antagonist ketanserin was administered after each DOI dose-response curve. All drugs were administered intravenously.Results: Compared to controls, comprehensive urodynamic studies demonstrated DM rats had a higher bladder capacity and residual volume, anda markedly reduced voiding efficiency. In DM rats，DOI (0.01 to 0.3 mg/kg)induced significant dose dependent decreases in residual volume ， and increases in micturition volume ,resulting in increased voiding efficiency.英文摘要5-羟色胺 2A/2C 受体激动剂 DOI 改善糖尿病大鼠排尿障碍的实验研究CMG measurements showed a dose-dependent increase in high-frequency oscillations(HFOs) activity, evident as an increased duration of HFOs per