a型禽流感病毒分子模式分析 word格式.docx

对两株新分离H5N1 亚型禽流感毒株进行了基因组序列分析，并采用跨宿主传播表型相关联的模式分类方法，对两株病毒进行了预测。结果显示，两株病毒为N类群，不具备感染人的能力。对两株病毒的分析，是分子模式研究的应用。关键词：A型禽流感病毒，基因组，小波包，层次聚类，H5N1 亚型AbstractH5N1viruseswerecontinuouslyreportedtobeepidemicfrom2003andcausedserious economicloss,towhichmuchsocialattentionhasbeenpaid.AvianinfluenzaAviruses coulddirectlyinfecthumansandothermammals.Althoughavianinfluenzavirusescouldnotspreadamonghumans,theyhadpartlyadaptedhumansandmammalsandcoulddeducepandemics.Thedifferencesbetweenthesequencesofavianinfluenzavirusesarevaluabletobestudied.Themolecularcharacterizationsofavianinfluenzavirusesisolatedfromhumansareveryimportant.Inthisresearch,thegenomesequencesof239influenzaAviruseswithdifferentsubtypesweretransformedandclassifiedtofindthepatternsassociatedwiththephenotypeofinterspeciestransmission.Theenergyfeatureswereextractedbythemethodofwaveletpacketdecompositionandused for virus classification by themethodofhierarchicalclustering.TheclusteringresultsshowedthatfivepatternsexistinavianinfluenzaA viruses,whichassociatedwiththephenotypeofinterspeciestransmission,andthatavianviruseswithpatternsCandE（YCluster）couldacrossspeciesbarrierandthosewithpatternsA,BandD（NCluster）mightnothavetheabilities.Based on the Euclideandistances，39 key sits werefounded. The 39 sitesare conservedwithinindividualpatternsandvariedamongpatterns.13keysiteslocatedinNS1,whichsuggestedthatNS1mutatedfastest.Moreover,thelowsimilaritysequencesinnatural‘genereservoir’werereconstructedwiththemethodofthemaximumlikelihood.ExceptM1andNS1,fourotherinnerproteinshadsmallclustersandPB2hadYclusterforashort time.ItwassuggestedthattheinterspeciestransmissionofavianinfluenzaAvirusesisanemergence.Thereasonisthattherangesofhumanactivitiesexpandedandthechanceof thecontactsbetweenvirusesandhumansareadded.Virusescouldspreaddirectlytoward thenewhosts.Twopredictionsystemswereconstructed:1)ThepredictionsystemsofmolecularpatternsofavianinfluenzaAviruswereconstructedbasedonKNNmodel.Theaccuracywas100%.Thissystemcouldbeausefulcomplementforearly