赤霉素调控半夏块茎形成-植物学专业论文 word格式.docxVIP

libraries,obtained300validESTs,ofwhich18ESTwereunknownfunction sequencethroughtheBlastanalysis,theremainingESTcaninfertheirhomologous genesorproteinfunctions.Theobtainedsequenceinvolvesaseriesofbiological processofcarbohydratemetabolism,proteinmetabolism,energymetabolism,DNA transcriptionandtranslation,signaltransduction,membranetransport,cellstructure andcelldivisionandgrowth.ThewholeESTswereclassifiedwith2levelsinthe formofcellcomponents,biologicalprocessandmolecularfunctionviaGO classificationstandard.3.5specificESTsequencesrespectivelyhavethehighergenehomologywith the60Sribosomalprotein(60SribosomalproteinPtRSP60),the26SribosomalRNA gene(26SribosomalRNAgene,PtRAR26),zinctransporterprotein(thetheZinc transporter,PtZT),12KDprotein(12kDstorageprotein,PtSP12),malate dehydrogenase (malate dehydrogenase, PtMDH) in the library. Analyse the expressionmodeofthese5genesthroughthequantitativePCRtechnology,andthe resultsshowedthattheexpressionqualityofPtRSP60geneandothersgeneschange significantly.It’s speculatedthattheymaybeassociatedwithmicrotuberformation. Keywords：Pinellia ternate(Pt);gibberellin;chlormequat chloride chlorocholine (CCC);invitrotubers;suppression subtractivehybridization(SSH)缩略语表（Abbreviations）简写符号英文全称中文全称AmpAmpicillin氨苄青霉素MSMurashigeandSkoog(1962)MS基本培养基ddH2ODoubledistilledwater双蒸水rpmRevolutions perminute每分钟转速BLASTBasiclocalalignmentsearchtool局部比对基本检索工具cDNAComplementary DNA互补DNADEPCDiEthyPyroCarbonate焦碳酸二乙酯RNARibonucleicacid核糖核酸ds cDNADoublestrandcDNA双链互补DNAESTExpressedsequencetag表达序列标签IPTGIsopropylthio-β-D-galactoside异丙基硫代-β-D-半乳糖苷LBLuria-BertaniLB培养基NCBINationalCenterforBiotechnology Information美国国立生物技术信息中心CCCchlormequatchloride矮壮素DNADeoxyribonucleicacid脱氧核糖核酸GA3Gibberellicacid赤霉素反转录/实时定量聚合酶RT-PCRReversetranscript/RealtimePCR链式反应SSHSuppressionsubtractivehybridization抑制消减杂交X-gal5-bromo-4-chloro-3-indol-β-D-galactocide5-溴-4-氯-3-吲哚-β-D-半乳糖苷目录第一章文献综述及研究背景........................................................................................11.1变态器官