清热益气化瘀通络复方对糖尿病肾病大鼠足细胞表型转化的作用研究-中西医结合;临床专业论文 word格式.docxVIP

（P0.05）；中药组与厄贝沙坦组间比较无统计学差异（P0.05）。⑤DES：DN模型组肾小球阳性信号面积比、积分光密度、mRNA 表达、相对定量最高，正 常对照组最低，中药组、厄贝沙坦组低于 DN 模型组（P＜0.05）；高于正常对照 组，但无统计学差异（P0.05）；中药组与厄贝沙坦组间无统计学差异（P0.05）。⑥相关性分析：24h 尿蛋白定量与 WT1 积分光密度无相关性（r＝-0.337, P0.05）， 与 P-Ca 积分光密度呈负相关（r＝-0.600, P0.05）；与 α-SMA 肾小球内积分光密 度呈正相关（r＝0.420, P0.05），与 DES 肾小球内积分光密度呈正相关（r＝0.750,P0.01）。结论：①早期 STZ 造模 DN 大鼠（12 周）足细胞数目尚无明显减少时， 尿中蛋 白已明显增加；②伴随此期 DN 蛋白尿发生，足细胞 P-Ca 的表达降低、α-SMA 及 DES 的表达升高；并且，尿蛋白含量与足细胞 P-Ca 的表达降低、α-SMA 及 DES 的表达升高有相关性；③DN 大鼠（12 周）足细胞可能存在 EMT；④清热 益气化瘀通络复方可降低 DN 大鼠 24h 尿蛋白定量，并增加足细胞 P-Ca 的表达 而降低 α-SMA 及 DES 的表达，显示具有拮抗 DN 大鼠 EMT 的作用。关键词：糖尿病肾病足细胞表型转化 上皮－间充质细胞转分化 清热益气化瘀通络复方AbstractObjective: To assess the effects of Qingreyiqi and Huayutongluo mixture on podocyte epiithelial-to-mesenchymal transition(EMT) in diabetic nephropathy(DN)rats．Methods：Male Wistar rats were randomly divided into four groups: control group(N, n=7), diabetic nephropathy group(DN, n=6), treated by Qingreyiqi and Huayutongluo mixture group(Q, n=6) and treated by Irbesartan group(I, n=6). After 12 weeks, itsblood gloucose, blood creatine, BUN and 24-hour urine protein were determined through bio-chemical analysis, and the expressions of P-Cadherin,WT1,α-SMA and desmin were observed by immunohistochemistry staining and real-time RT-PCR.Results：①Compared with N group, the blood gloucose increased in rats of other groups（P0.05）. The blood creatine and BUN between the four groups had no difference（P0.05）. The 24-hour urine protein increased in DN rats（P0.05）, butQ group and I group decreased noticeably and the Q group decreased more lowly（P0.05）②The positive area ratio, integral optical density, mRNA expression and relative quantification of WT1 had no differences between four groups（P0.05）. ③The positive area ratio, integral optical density, mRNA expression and relative quantification of P-Cadherin were decreased progressively in DN（P0.05）, but in Q and I groups, they all increased compared with DN（P0.05） , and had no differences with N group （P0.05）except the pos