应用原子力显微镜分析正常淋巴细胞和Jurkat细胞的形态和机械性质.PDF

生 物 工 程 学 报 Chin J Biotech 2009, July 25; 25(7): 1107-1112 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2009 Institute of Microbiology, CAS CSM, All rights reserved 生物技术与方法 Jurkat 蔡小芳, 蔡继业, 董世松, 邓华, 胡明铅 , 510632 : 淋巴细胞形态和机械性质的变化与人的健康、疾病的治疗和诊断有着密切关系。本研究利用原子力显微镜研 究淋巴细胞和Jurkat 细胞形态和机械性质。结果显示, 这2 种细胞的形态较为相似, 但通过对力曲线的分析得出这2 种 细胞的机械性质明显不同。正常淋巴细胞粘弹力范围大致为(796.7±248.5) pN, 而Jurkat 细胞分布于(158.5±37.5) pN；正 常淋巴细胞的杨氏模量(0.471 kPa±0.081 kPa)近4 倍于Jurkat 细胞(0.0964 kPa±0.0229 kPa)；而Jurkat 细胞(4.322 mN/m± 0.382 mN/m) 的硬度近2 倍于正常淋巴细胞(2.278 mN/m±0.488 mN/m) 。结果表明原子力显微镜能可在临床诊断上区分正 常细胞与肿瘤细胞, 即使两者形态区别不明显。 : 原子力显微镜, Jurkat 细胞, 正常淋巴细胞, 表面形态, 机械性质 Morphology and mechanical properties of normal lymphocyte and Jurkat revealed by atomic force microscopy Xiaofang Cai, Jiye Cai, Shisong Dong, Hua Deng, and Mingqian Hu Department of Chemistry , Jinan University, Guangzhou 510632, China Abstract: Alternations of lymphocyte in biophysical properties (e.g., morphology and viscoelasticity) are related to the human health, disease diagnosis and treatment. Here, we used atomic force microscopy (AFM) to characterize the morphology and mechanical properties of normal lymphocyte and Jurkat. The AFM images revealed that their cell shapes appeared similar. The mechanical properties of the two groups were tracked with AFM-based force spectroscopy. The normal lymphocyte cells had a high adhesion force distribution in (796.7±248.5) pN, whereas the Jurkat