甜樱桃根际IAA产生菌的筛选鉴定及最佳产素条件优化1赵柏霞12.DOCVIP

甜樱桃根际IAA产生菌的筛选、鉴定及最佳产素条件优化 赵柏霞1,2 刘浩强3孙丽娜1 肖敏1 郑玮1 赵慧1 潘凤荣1* (1大连市农业科学研究院，辽宁 大连 116036；2 沈阳农业大学 土地与环境学院 辽宁 沈阳 110866； 3中国农业科学院柑桔研究所 重庆 400712） 摘要：为了给甜樱桃微生物肥料的研制和生产提供优良菌株资源。本文从马哈利樱桃根际土壤中分离获得52株细菌，通过保绿法和萝卜子叶增重法进行IAA产生菌的筛选，并经形态观察、生理生化特征及16S rDNA序列分析明确分类地位，利用分光光度法测定产IAA的能力，并对最佳碳氮源、产素条件进行了筛选。结果表明52株菌中B20产IAA能力最强，经鉴定为阿氏芽孢杆菌（Bacillus aryabhattai），其最佳产素的培养条件为：采用10%的装液量在温度为30℃、转速为150 r· min-1的条件下培养4h，最佳碳、氮源分别为果糖和酵母粉。研究发现菌株B20具有IAA产生能力，具有作为功能微生物肥料研发的潜力，可进一步对其进行开发研究。 关键词：吲哚乙酸（IAA）；根际；筛选；鉴定；条件优化 Screening and identification of IAA biosynthesis strain of rhizobacteria from cheery and optimization of its culture conditions ZHAO Baixia1,2, LIU Haoqiang3, SUN lina1, XIAO min1, ZHENG wei1, ZHAO hui1, PAN fengrong1* (1 Dalian Academy of Agriculture Sciences, Dalian，Liaoning 116036; 2 Land and Enviromental Sciences, Shenyang Agricultural University, Shenyang, Liaoning 110866; 3 Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712) Abstract：The paper aimed at providing the excellent strains for the development and production of cheery microbial fertilizer. 52 bacteria strains were isolated and purified from the rhizosphere soil of Mahaleb. A mass screening was carried out using the methods of remaining green and radish cotyledon weight dioassay. Further, IAA biosynthesis strains were screened. And the strains were identified through the morphological feature and 16S rDNA sequence analysis. Among them, strain B20 was the highest in IAA excreting capability. It was identified as Bacillus aryabhattai. The conditions for the strain growth and fermentation were optimized by measuring IAA content. The optimal culturing conditions for both growth and IAA secretion were filling volume 10%, incubation at 37℃ for 44h and shaking speed 150 r· min-1, fructose and Yeast powder as carbon source and nitrogen source respectively. The strain B20 is expected to be excellent microbial fertilizer. Key words indole acetic acid; rhizosphere; screening; identification; optim