蝉蜕活性成分的提取及其抑菌活性的研究.pdf

昆虫知识 Chinese Bulletin of Entomology 20 10 ，47 (6 ):1109 ～ 1112 蝉蜕活性成分的提取及其抑菌活性的研究* 王 珏 田强强 陶 刚 高秋荣 吕婷婷 王 敦 ( 7 12 100 ) 植保资源与病虫害治理教育部重点实验室西北农林科技大学 杨凌 Analyses on ingredients and antibacterial activity of periostracum cicadae. WANG Jue ，TIAN Qiang-  Qiang ，TAO Gang ，GAO Qiu-Rong ，LV Ting-Ting ，WANG Dun (Key Laboratory of Plant Protection Resources and Pest M anagement (Northwest A ＆F University )，M inistry of Education ，Northwest A ＆F University ，Yangling 7 12 100 ，China) Abstract The antimicrobial components of the traditional Chinese herbal medicine ，periostrcum cicadae were extracted and their antibacterial activity examined . Four extraction methods and seven extraction solvents were used . The results show that the highest yield was produced by ultrasonic extraction with 95 % ethanol . The antibacterial activity of the obtained extracts against Escherichia coli was determined using different bioassays. The bioassay results indicate that all extracts had an obvious inhibitory effect on E. coli compared to the control (P ≤0. 05 ). There was no significant difference in antimicrobial activity between different extracts. The highest antimicrobial activity was from extracts obtained by ultrasonic extraction with 95 % ethanol ，the minimum inhibitory concentration (MIC)of this extract on E. coli was 0. 078 mg / mL . Key words periostrcum cicadae ，bioactive component ，antibacterial activity ，extraction 。 4 7 摘 要 本文报道了对传统中药蝉蜕抑菌活性成分提取与抑菌活性研究结果 对 种提取方法和 种 ， ， 95 % ，4 提取溶剂的提