棉花C2H2类型锌指蛋白基因1的克隆及表达分析.PDFVIP

棉 花 学 报 Cotton Science 20 15袁27 3 冤院189―197 棉花C2H2 类型锌指蛋白基因 1 的克隆及 表达分析 新宇 林书岱 张涛 裴柳玲 唐清 刘峰 刘永昌* 河子大学农学院/ 新疆生产建设兵团绿洲生态农业重点实验室袁新疆石河子832000冤 摘要院通过RT-PCR 技术从陆地棉中克隆到1 个编码C2H2 型锌指蛋白的基因袁命名为 1(Stress Induced Zinc finger protein1) 遥该基因编码239 个氨基酸残基袁预测蛋白分子量为26.6205 kD 袁等电点为6.52遥GhSIZ1 包 含一个C2H2 型锌指结构域袁在其N 端含有保守的L-box 袁C –末端含有一个转录抑制结构域EAR/DLN-box 遥 系统发育树分析表明GhSIZ1 与可可 XP_007044496.1冤和中粒咖啡 CDP002 18.1冤中的锌指蛋白亲缘关系较 遥实时定量PCR 分析 1 在棉花各组织中均有表达袁但在根中表达量最高遥低温尧高盐尧干旱胁迫处理后 1 的表达水平显著上调遥 亚细胞定位分析表明袁GhSIZ1 定位在细胞核中遥 本研究表明袁 1 可能在棉 花响应低温尧高盐尧干旱等非生物胁迫中起着重要作用遥 关键词院棉花曰C2H2 型锌指蛋白曰表达模式曰 1 中图分类号院S562.035 文献标志码院A 文章编号院1002-7807 20 15冤03-0 189-09 10.11963/issn.1002-7807.201503001 Zhang Xinyu, Lin Shudai, Zhang Tao, Pei Liuling, Tang Qing, Liu Feng, Liu Yongchang* ( 832000, ) In this study, a novel gene encoding C2H2-type zinc finger protein was cloned in cotton by reverse-transcription poly- merase chain reaction (PCR). The gene was designated as 1 (Stress-induced Zinc finger protein 1). 1 encoded a pro- tein of 239 amino acid residues with a predicted molecular mass of 26.6205 kD and an isoelectric point of 6.52. It has a C2H2-type zinc finger domain, an L-box at the N terminus and a putative transcription repression domain(EAR/DLN-box) at the C-terminus. Phylogenetic analysis revealed that 1 shares high identity with C2H2-type zinc finger proteins from (XP_007044496.1) and (CDP00218.1). Real-time PCR analysis showed that the transcripts of 1 were detected in all tissues: roots, stems, flowers, leaves and fibers. The highest level was found in roots. The expression w