低温纤维素酶菌株CNY086选育及发酵培养基优化Ⅰ.PDFVIP

微生物学通报 OCT 20, 2009, 36(10): 1547~1552 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 低温纤维素酶菌株 CNY086 选育及 发酵培养基优化( Ⅰ) 陈 亮 迟乃玉 张庆芳* ( 116622) 摘 要 : 自渤海湾海泥中分离 21 株低温纤维素酶产生菌。其中菌株 CNY01 为绿色木霉 (Trichoderma viride), 酶活力为67.30 U/mL 。以该菌株为出发菌株, 经UV 、DES 等诱变, 选育出 高产突变菌株CNY086, 酶活力为92.17 U/mL 。该突变菌株低温纤维素酶发酵具有遗传稳定性。 通过单因素和正交实验确定突变菌株 CNY086 低温纤维素酶发酵最适培养基: 秸秆粉 1.20%、麸 皮0.70% 、硫酸铵0.50% 、磷酸二氢钾0.55%, 上述条件下CNY086 菌株酶活力达到108.55 U/mL。 关键词: 低温纤维素酶, 选育, 优化 Breeding and Fermentation Medium Optimization of Cold-active Cellulase Strain CNY086 ( Ⅰ) * CHEN Liang CHI Nai-Yu ZHANG Qing-Fang (College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China) Abstract: 21 cold-active strains which produced cold-active cellulase were isolated from Bohai Bay. One of the strains CNY01 was a kind of Trichoderma viride and its cellulase activity was 67.30 U/mL. Through UV and DES mutation, the strain CNY086 was bred and its cellulase activity was 92.17 U/mL. The strain CNY086 had stable ability to produce cellulase. Through orthogonal experiment, the optimal condition of the fermentation medium had been tested. The suitable medium was that straw power 1.20%, bran 0.70%, (NH ) SO 0.50%, KH PO 0.55%, in this condition the cellulase activity came to 108.55 U/mL. 4 2 4 2 4 Keywords: Cold-active cellulase, Breeding, Optimization , 1.5