抗炎受试物A对溃疡性结肠炎大鼠模型的影响-药学毕业论文推荐.docVIP

本科生毕业论文（设计） 抗炎受试物A对溃疡性结肠炎大鼠模型的影响 [论文摘要] 背景：炎症性肠病（IBD，Inflammatory bowel disease）是病因尚未明确的慢性非特异性肠道炎症性疾病，包括溃疡性结肠炎（UC，ulcerative colitis）和克罗恩病(CD，Crohn’s disease)。该病在西方国家比较多见，但近年在我国的发病率亦有上升趋势。因其病因不明，一直是消化科较为棘手的疾病。目的：本论文实验主要针对溃疡性结肠炎（UC），研究抗炎受试物A对溃疡性结肠炎大鼠模型的治疗作用及其可能的作用机制。方法：实验造模由50只SD雄性大鼠，先分为正常组10只，模型组40只，模型组大鼠用塑胶管由肛门轻缓插入大鼠体内深约8 cm，按照100 mg/kg的剂量缓慢注入含三硝基苯磺酸TNBS(trinitro-benzene-sulfonic acid) 25g/L的乙醇溶液，建立大鼠溃疡性结肠炎模型。造模成功的大鼠按体重随机分为4组，每组10只，分别为正常组，模型对照组，受试物低剂量组，受试物高剂量组，美沙拉嗪控释颗粒组，每天1次分别灌胃给药，连续10 d。每天进行大鼠日常和体重观察，实验结束后取病变结肠组织病理检查，测定其MPO酶活性。结论：最后得出，抗炎受试物能降低MPO含量，显著性降低溃疡性结肠炎的病理评分（P0.05） [关键词] 炎症性肠病；抗炎受试物A；溃疡性结肠炎；三硝基苯磺酸；MPO酶活性  The influence of Mesalazine in ulcerative colitis rats [Abstract] Inflammatory bowel disease is an nonspecific Chronic Inflammation of the bowel, and it is not yet clear about Causes of illnes. This illness Including ulcerative colitis and Crohn’s disease, and it is more common in western countries, however, the trend of Incidence infection is increasing in these years in our country. And The illness has been a hot potato in Digestion section for a long time. This Paper experiment mainly at ulcerative colitis, Research by test compound to investigate the effect of ulcerative colitis(rats. Molding methods 50 SD rats, the plastic tube insert rat’s body 8cm from fundament, with 100 mg/kg instill TNBS of 25g/L Surelease, then this is the model of ulcerative colitis rats. According to Body Weight , Made by successful SD rats were randomly divided into 5 groups: normal control group, model control group ，test compound high control group, test compound lower control group and Mesalazine positive control group, 10 rats a group, respectively through stomach for one times a day for 10 d . observe carefully the situation and Weight, for the organization of the colon to pathological examination after the paper experiment over. The results showed Subject matter can reduce the content of MPO, and reduce Pathological mark of UC markedly. [Keywords] Inflammator