创新型试验教学项目建设的探索——土壤微生物分离试验.PDF

微生物学通报 Dec. 20, 2014, 41(12): 2525−2529 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140201 高校教改纵横 创新型实验教学项目建设的探索——土壤微生物分离实验 夏曦中 彭方 车婧 李文化 陈向东 谢志雄* ( 430072) 摘 要武汉大学生命科学学院微生物学实验教学中注重创新型实验教学项目的建设，创新型 土壤微生物分离实验项目建设是培养学生创新能力的一次实践。从武汉大学校园和国家级自然 保护区神农架采集土壤样品，分离土壤细菌、放线菌和霉菌。让学生了解土壤微生物数量测定 的基本方法，学习了解平板计数的基本操作技术。在引申实验过程中又有机地结合与贯穿了微 生物学的无菌操作技术、显微镜技术、纯培养技术、分离纯化技术等各项技术及最新的分子生 物学研究方法和技术。本实验的创新点有三：首先，实验从室内延伸到室外；其次，从本科生 的常规土壤微生物的分离实验衍生到科研实验(新菌种的分离、鉴定)；最后，使共性教学衍生 为个性化培养。 关键词创新型实验，土壤微生物分离，神农架 Exploration on innovative experimental item construction for isolation of microorganisms in soil * XIA Xi-Zhong PENG Fang CHE Jing LI Wen-Hua CHEN Xiang-Dong XIE Zhi-Xiong (College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China) Abstract: It is a practice that we foster students’ innovation ability and explore innovation experiment in microbiology experimental teaching. Students were assigned to isolation of bacteria, actinomycetes and molds from campus’s soil and Shennongjia’s soil, which they had collected during summer filed practice. The experiment is aimed at helping students to learn the method for determination of microorganism numbers in soil and master the basic techniques about plate count. The subsequent experiments involve techniques, such as aseptic technique, microscopical technique, pure culture technique, separation and purificati