梨叶多酚提取的正交试验优化及其成分测定-河南农业科学.PDF

32 2015, Vol.36, No.20 食品科学 ※工艺技术 梨叶多酚提取的正交试验优化及其成分测定 * 郑迎春，曹玉芬 ，李 静，田路明，董星光，张 莹，齐 丹，常耀军 （中国农业科学院果树研究所，农业部果品质量安全风险评估实验室（兴城），辽宁兴城 125100 ） 摘  要：以早酥梨成熟叶片为研究对象，考察超声时间、料液比、溶剂种类3 个因素对梨叶多酚提取效果的影响， 以优化梨叶片多酚提取工艺，并采用超高效液相色谱-光电二极管阵列技术，测定梨叶片多酚物质组成和含量。结 果表明：梨叶片中熊果苷最佳提取条件是：采用70% 甲醇溶液提取溶剂、超声（超声功率500 W ）辅助提取2 次、 每次超声时间30 min 、料液比1 ∶150 （g/mL ）；梨叶片中咖啡酰奎宁酸类和黄酮醇类的最佳提取条件均是：超声 （超声功率500 W ）辅助提取2 次、每次超声时间25 min、采用70% 甲醇溶液提取溶剂、料液比1∶125 （g/mL ）。在 优化的提取条件下，梨叶中共检测出10 种多酚物质，其中熊果苷含量最高为7.934～36.854 mg/g，被检测出的多酚 物质总含量高达17.852～61.149 mg/g，因此梨叶片可作为开发化妆品、保健品和药品的优质天然材料。 关键词：早酥梨叶；超高效液相色谱；多酚；提取；成分 Optimization of Extraction of Pear Leaf Polyphenols by Orthogonal Array Design and Polyphenol Composition Analysis * ZHENG Yingchun, CAO Yufen , LI Jing, TIAN Luming, DONG Xingguang, ZHANG Ying, QI Dan, CHANG Yaojun (Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment for Fruit (Xingcheng), Ministry of Agriculture, Xingcheng 125100, China) Abstract: The ultrasonic-assisted extraction of polyphenols from mature leaves of Zaosu pear was optimized by investigating the effects of ultrasonic time, solid-to-solvent ratio and solvent type on extraction efficiency. The components and contents of pear leaf polyphenols were determined by ultra performance liquid chromatography (UPLC) coupled with photodiode array detector (PDA). The results showed that the optimal conditions for arbutin extraction from pear leaves were found to be 30 min extraction carried out twice using 70% aqueous ethanol as the extraction solvent with a solid-to-solvent ratio of 1:150 (g/mL) at an ultrasonic power of 500 W, while the optimal cond