蒲地蓝消炎口服液对头孢克洛在大鼠肠道吸收的影响.PDFVIP

- 170 - Drug Evaluation Research 第 38 卷 第 2 期 2015 年 4 月 蒲地蓝消炎口服液对头孢克洛在大鼠肠道吸收的影响 1, 2 3 1* 罗轶凡 ，孙耀庭 ，姜明燕 1. 中国医科大学附属第一医院药学部，辽宁 沈阳 110001 2. 中国医科大学药学院，辽宁 沈阳 110001 3. 大连医科大学药学院，辽宁 大连 116044 摘 要：目的 建立小肠灌流液中头孢克洛的高效液相色谱（HPLC ）分析方法，考察蒲地蓝消炎口服液对头孢克洛在大鼠 小肠吸收的影响，为临床联合用药的合理性提供实验依据。方法 采用大鼠在体单向肠灌流实验模型，应用重量法校正灌流 液体积，HPLC 测定灌流液中头孢克洛，计算头孢克洛肠吸收参数。结果 头孢克洛在各肠段均有良好吸收，小肠不同部位 头孢克洛的吸收速率常数（Ka ）与有效渗透系数（Peff ）大小为十二指肠＞空肠≈回肠，各肠段间的吸收无统计学差异；联 合应用蒲地蓝消炎口服液时，头孢克洛在全场段的吸收均有所减少，十二指肠处显著减少（P ＜0.05 ），其他肠段处无统计学 差异（P ＞0.05 ）。结论 研究结果表明蒲地蓝消炎口服液会影响头孢克洛的小肠吸收，因此两药联合使用时产生较好的药效 不是通过促进头孢克洛的吸收而实现的，而是二者共同作用的结果。 关键词：蒲地蓝；头孢克洛；小肠吸收；单向肠灌流 中图分类号：R969.2 文献标志码：A 文章编号：1674 - 6376 (2015) 02 - 0170 - 05 DOI: 10.7501/j.issn.1674-6376.2015.02.011 Effects of Pudilan on absorption of cefaclor in small intestine of rats 1, 2 3 1 LUO Yi-fan , SUN Yao-ting , JIANG Ming-yan 1. Department of Pharmacy, the First Affiliated Hospital of China Medical University, Shenyang 110001, China 2. School of Pharmacy, China Medical University, Shenyang 110001, China 3. College of Pharmacy, Dalian Medical University, Dalian 116044, China Abstract: Objective To establish a method for the determination of cefaclor by HPLC and to investigate the effects of Pudilan on intestinal absorption of cefaclor in rats. Methods The rat single-pass intestinal perfusion technique was applied to investigatting the intestinal absorption of cefaclor, and the gravimetry was used to correct the perfusion volume. After the concentration of cefaclor in perfusate was determined by HPLC, the absorption parameters of cefaclor were calculated. Results Cefaclor was absorbed well in all parts of the small intestine. The absorption rate constants (Ka) and the effective permeability coefficients (Peff) of cefaclor were ordered as follows: duodenum jejunum ≈ ileu