非洲马铃果果籽提取物的体外抗肿瘤活性-亚热带植物科学.pdfVIP

2017,46(2): 113 ～116. Subtropical Plant Science 非洲马铃果果籽提取物的体外抗肿瘤活性 1 1 2 2 周 晶 ，刘佳佳 ，吴义强 ，袁广明 (1. 中南大学化学化工学院医药生物化工研究所，湖南 长沙 410083 ；2. 中南林业科技大学，湖南 长沙 410004) 摘 要：分别以不同极性有机溶剂(正己烷、二氯甲烷、乙酸乙酯、丙酮与乙醇)对非洲马铃果的果籽进行索氏 提取，测定提取率；以人宫颈癌细胞(Hela Cells)与人肝癌细胞 ( SMMC-7721 细胞株)为对象，对 5 种有机相的 非洲马铃果果籽提取物进行体外细胞毒理性研究，测定提取物抗肿瘤活性。结果表明，马铃果果籽丙酮提取物 对 SMMC-7721 癌细胞具有显著的增殖抑制活性，同时也表现出对 Hela 癌细胞较高程度的抑制作用；正己烷 m 提取物对 Hela 癌细胞具有相对较明显的抑制活性，对 SMMC-7721 癌细胞药理活性不显著；其他有机相提取 物几乎没有肿瘤细胞增殖抑制活性。 o 关键词：非洲马铃果；提取物；细胞毒理活性 Doi: 10.3969/j.issn.1009-7791.2017.02.003 c 中图分类号：Q949.776.5 文献标识码：A 文章编号：1009-7791(2017)02-0113-04 . Antitumor Activity of Extracts from Voacanga africana Seeds x 1 1 2 2 ZHOU Jing , LIU Jia-jia , WU Yi-qiang , YUAN Guang-ming k (1.Institute of Pharmaceutical Biochemistry, School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan China; 2. Central South University of Forestry and Technology, Changsha 410012, Hunan China) w Abstract: With different polarity organic solvents (n-hexane, dichloromethane, ethyl acetate, acetone and ethanol), extraction yield of Voacanga africana seeds by Soxhlet extraction were determinated. z The inhibitory activity of extracts against human tumor cell proliferation was tested using Hela Cells and SMMC-7721 Cells as the experimental object and the MTT method as judge standards. The