低剂量微波辐射诱导的γ射线致小鼠遗传损伤的适应性反应分析-卫生毒理学专业论文.pdf.docxVIP

低剂量微波辐射诱导的 γ 射线致小鼠遗传损伤的适应性反应研究中文摘要导的小鼠骨髓及外周血细胞微核细胞率。3. 氧化应激途径很有可能参与了低剂量微波诱发小鼠适应性反应，减轻 γ 射 线致遗传物质损伤的过程。关键词：微波、γ 射线、适应性反应、辐射、单细胞凝胶电泳、微核、ROS、SOD作者：姜秉成 指导教师：曹毅 副研究员英文摘要低剂量微波辐射诱导的 γ 射线致小鼠遗传损伤的适应性反应研究Adaptive Response Induced by Low Dose Microwave on Genetic Damages of Mice Caused by γ-raysAbstractObjective：To explore the adaptive response induced by low dose microwave on γ-rays-caused genetic damages in mice.Methods:Adult male ICR mice were pre-exposed to 900 MHz radiofrequency radiation at 120μW/cm 2 power density for 4 hours/day for 1, 3, 5, 7 and 14 days and then subjected to an acute dose of 3 Gy γ-radiation. The primary DNA damage in the form of alkali labile base damage and single strand breaks in the DNA of peripheral blood leukocytes was determined using the alkaline comet assay.Adult male ICR mice were pre-exposed to non-ionizing radiofrequency fields, 900MHz at 120μW/cm 2 power density for 4 h/day for 7 days and then subjected to an acute whole body dose of 3 Gy γ-radiation. The classical micronucleus assay was used to determine the extent of genotoxicity in immature erythrocytes in peripheral blood and bone marrow.Adult male ICR mice were pre-exposed to non-ionizing radiofrequency fields, 900MHz at 120μW/cm 2 power density for 4 h/day for 7 days and then subjected to an acute whole body dose of 3 Gy γ-radiation. DCFH-DA was used to study the content of reactive oxygen species. The vitality unite of superoxide dismutase was measured with total superoxide dismutase assay Kit.Results:The results of alkaline comet assay indicated that the extent of damage in mice which were pre-exposed to radiofrequency radiation for 1 day and then subjected to γ-radiation was not significantly different from those exposed to γ-radiation alone. However, mice which were pre-exposed to RF for 3, 5, 7 and 14 days showed低剂量微波辐射诱导的 γ 射线致小鼠遗传损伤的适应性反应研究 英文摘要 progressively decreased damage and was significantly different from those exposed to γ-radiation alone. Thus, the d