碘海醇对大鼠肾草酸钙结石形成影响的实验研究-外科学专业论文.pdf.docxVIP

碘海醇对大鼠肾草酸钙结石形成影响的实验研究摘要目的研究不同剂量碘海醇对大鼠肾草酸钙结石形成的影响。方法将60只Wistar大鼠随机分为成6组：空白对照组(A)、单纯诱石组(B)、单纯碘海醇组(C)、诱石＋小剂量碘海醇组(D)、诱石＋普通剂量碘海醇组(E)、诱石＋大剂量碘海醇组(F)。用乙二醇饮水法诱导建立肾草酸钙结石动物模型。C、D、E、F组给予尾静脉注射碘海醇。实验第4周末处死大鼠，取双侧肾脏，左肾HE染色石蜡切片，光镜下观察肾组织草酸钙结晶沉积及病理改变情况，按文献标准分级评分；右肾制备组织匀浆，检测肾组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力。结果与A组相比，B、D、E、F组结晶等级评分高于A组，差异有统计学意义(P0.05)；F组结晶等级评分高于B组，差异有统计学意义(P0.05)；B、D、E三组间结晶等级评分差异无统计学意义(P＞0.05)。A、B、C、D、E、F六组肾脏组织MDA 含量分别为(2.74±0.48)、(4.51±1.47)、(4.77±1.58)、(6.03±1.57)、(7.44±1.44)、(8.87±2.31)nmol/mg蛋白，SOD活力分别(195.21±11.01)、(170.24±13.59)、(169.57±13.30)、(154.67±13.99)、(138.39±22.63)、(121.54±25.50)U/mg 蛋白，组间差异有统计学意义(P0.05)。结论①三种剂量的碘海醇均对草酸钙结石大鼠的肾脏造成了氧化损伤，损伤程度与碘海醇剂量呈正相关。②大剂量重复注射碘海醇促进了肾小管内草酸钙结晶量的增加，此过程与碘海醇诱导的肾小管上皮氧化应激损伤有关。关键词肾结石；草酸钙；碘海醇；造影剂肾病；氧化应激EffectsofiohexolonexperimentalcalciumoxalatecrystallizationformationinratsAbstractObjectiveToinvestigatetheeffectsofdifferentdoseof iohexolinjectedonexperimentalcalciumoxalatecrystallizationinrats.MethodsAtotalof60ratswererandomlydividedinto6groups:blankcontrolgroup(A),stoneforminggroup(B),iohexolgroup(C),stoneformingandsmalldoseiohexolgroup(D),stoneformingandmediumdoseiohexolgroup(E),stoneformingandlargedoseiohexolgroup(F).Calciumoxalatecrystalswereproducedinratsbyethyleneglycol.After4weekstheratsweresacrificedandtherenaltissueswereremovedfordeterminationofmalonaldehyde(MDA)contentandsuperoxidedismutase(SOD)activities.Pathologicalchangesinrenaltissueswereobservedwithlightmicroscopes．ResultsComparedwithgroupA,thegradeofcalciumoxalatecrystallizationwassignificantlyincreasedingroupB,D,EandF.Differencebetweenthemwasstatisticallysignificant(P0.05).ComparedwithgroupB,thegradeofcalciumoxalatecrystallizationwassignificantlyincreasedingroupF.Differencebetweenthemwasstatisticallysignificant(P0.05).ThecontentofMDAinrenaltissueswere2.74±0.48,4.51±1.47,4.77±1.58,6.03±1.57,7.44±1.44,8.87±2.31nmol/mgprotein,andactivitiesof SODwere195.21±11.01,170.24±13.59,169.57±13.30,154.67±13.99,138.39±22.63,121.54±25.50U/mgprotein.Differencebetweenthemwasstatistical